- RMT3-23 (See other available formats)
- Regulatory Status
- Other Names
- T cell immunoglobulin and mucin domain containing 3 protein, hepatitis virus cellular receptor 2, CD366
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
|Cat #||Size||Price||Quantity Check Availability||Save|
CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on Th1 lymphocytes and CD11b+ macrophages. Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
- Excitation Laser
Violet Laser (405 nm)
- Application Notes
Additional reported applications (for relevant formats) include: in vitro1 and in vivo2 blocking of Tim-3, and immunohistochemical staining of frozen sections2. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 119731-119736).
(PubMed link indicates BioLegend citation)
- Nakae S, et al. 2007. Blood 110(7):2565-8. (FC, Block)
- Oikawa T, et al. 2006. J. Immunol. 177(7):4281-7. (FC, Block, IHC)
- Product Citations
AB_2716208 (BioLegend Cat. No. 119727)
- Transmembrane protein containing immunoglobulin domain and mucin-like domain; predicted molecular weight 31 kD; can exist as a soluble form lacking mucin and transmembrane domains
Expressed on activated Th1 lymphocytes and CD11b+ macrophages
- May play a role in the development of immune responses and the development of Th1-mediated responses
- Putative ligand on resting CD4+ lymphocytes
- Cell Type
- Macrophages, Th1
- Biology Area
- Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
1. Sabatos CA, et al. 2003. Nat. Immunol. 4:1102.
2. Sanchez-Fueyo A, et al. 2003. Nat. Immunol. 4:1102.
3. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454.
4. Mooney L, et al. 2002. Nature 415:536.
- Gene ID
- 171285 View all products for this Gene ID
- View information about CD366 on UniProt.org
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD366 (Tim-3)
PE anti-mouse CD366 (Tim-3)
APC anti-mouse CD366 (Tim-3)
PE/Cyanine7 anti-mouse CD366 (Tim-3)
Biotin anti-mouse CD366 (Tim-3)
PerCP/Cyanine5.5 anti-mouse CD366 (Tim-3)
GoInVivo™ Purified anti-mouse CD366 (Tim-3)
Brilliant Violet 605™ anti-mouse CD366 (Tim-3)
Brilliant Violet 421™ anti-mouse CD366 (Tim-3)
Brilliant Violet 711™ anti-mouse CD366 (Tim-3)
Brilliant Violet 785™ anti-mouse CD366 (Tim-3)
TotalSeq™-A0003 anti-mouse CD366 (Tim-3)
Ultra-LEAF™ Purified anti-mouse CD366 (Tim-3)
APC/Fire™ 750 anti-mouse CD366 (Tim-3)
TotalSeq™-C0003 anti-mouse CD366 (Tim-3)
TotalSeq™-B0003 anti-mouse CD366 (Tim-3)
Alexa Fluor® 647 anti-mouse CD366 (Tim-3)
PE/Fire™ 810 anti-mouse CD366 (Tim-3)
PE/Dazzle™ 594 anti-mouse CD366 (Tim-3)