- BC96 (See other available formats)
- Regulatory Status
- V T-072
- Other Names
- Low affinity IL-2R, IL-2R α chain, Tac, p55
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.Product Details
- Verified Reactivity
- Reported Reactivity
- Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
- Excitation Laser
Violet Laser (405 nm)
- Application Notes
Additional reported applications include: immunocytochemistry3.
(PubMed link indicates BioLegend citation)
- Product Citations
AB_11203536 (BioLegend Cat. No. 302633)
AB_2563807 (BioLegend Cat. No. 302634)
- Type I transmembrane glycoprotein, 55 kD
Activated T cells and B cells, monocytes/macrophages, Treg
- Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
- Cell Type
- B cells, Macrophages, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann T. 1991. J. Biol. Chem. 266:2681.
- Gene ID
- 3559 View all products for this Gene ID
- View information about CD25 on UniProt.org
Related Pages & Pathways
Other FormatsView All CD25 Reagents Request Custom Conjugation
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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APC anti-human CD25
FITC anti-human CD25
PE anti-human CD25
PE/Cyanine5 anti-human CD25
Purified anti-human CD25
APC/Cyanine7 anti-human CD25
PE/Cyanine7 anti-human CD25
Alexa Fluor® 488 anti-human CD25
Alexa Fluor® 647 anti-human CD25
Pacific Blue™ anti-human CD25
Alexa Fluor® 700 anti-human CD25
Biotin anti-human CD25
PerCP/Cyanine5.5 anti-human CD25
Brilliant Violet 421™ anti-human CD25
Brilliant Violet 605™ anti-human CD25
Brilliant Violet 650™ anti-human CD25
Brilliant Violet 711™ anti-human CD25
Brilliant Violet 785™ anti-human CD25
Brilliant Violet 510™ anti-human CD25
APC/Fire™ 750 anti-human CD25
TotalSeq™-A0085 anti-human CD25
PE/Dazzle™ 594 anti-human CD25
TotalSeq™-B0085 anti-human CD25
TotalSeq™-C0085 anti-human CD25
TotalSeq™-D0085 anti-human CD25