- PO3 (See other available formats)
- Regulatory Status
- Other Names
- B7-2, B70, Ly-58
- Rat IgG2b, κ
- Ave. Rating
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- Product Citations
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CD86 is an 80 kD immunoglobulin superfamily member, also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells and astrocytes. CD86 along with CD80 are the ligands of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells. The PO3 antibody has been shown to block autoantibody production in vivo and inhibit T cell activation in vitro.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- BALB/c mouse B leukemia cell line BCL1
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
- Excitation Laser
Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: in vivo and in vitro blocking 1,4,5 of autoantibody production and T cell activation, stimulation of B cell activity3, immunoprecipitation2, and immunohistochemical staining2 of acetone-fixed frozen sections.
(PubMed link indicates BioLegend citation)
- Nakajima A, et al. 1995. Eur. J. Immunol. 25:3060.
- Nuriya S, et al. 1996. Int. Immunol. 8:917.
- Kasprowicz DJ, et al. 2000. J. Immunol. 165:680.
- Saito K, et al. 1998. J. Immunol. 160:4225.
- Nakajima A, et al. 1998. J. Immunol. 161:1901.
- Ma XT, et al. 2006. Cancer Research 66:1169.
- Lawson BR, et al. 2007. J. Immunol. 178:5366.
- Bhatnagar S and Schorey JS. 2007. J. Biol. Chem. doi:10.1074/jbc.M702277200.
- Giroux M, et al. 2007. J. Immunol. 179:4492.PubMed
AB_2892271 (BioLegend Cat. No. 105125)
- Ig superfamily, 80 kD
B cells and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes
- T cell costimulation, Ig class-switching, NK cell cytotoxicity
- CD28, CD152 (CTLA-4)
- Cell Type
- Astrocytes, B cells, Dendritic cells, Macrophages, T cells, Tregs
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Hathcock KS, et al. 1993. Science. 262:905.
3. Freeman GJ, et al. 1993. Science. 262:907.
4. Carreno BM, et al. 2002. Annu Rev Immunol. 20:29.
- Gene ID
- 12524 View all products for this Gene ID
- View information about CD86 on UniProt.org
Related Pages & Pathways
Other FormatsView All CD86 Reagents Request Custom Conjugation
|PE anti-mouse CD86||PO3||FC|
|Purified anti-mouse CD86||PO3||FC,IHC-F,IP|
|FITC anti-mouse CD86||PO3||FC|
|APC anti-mouse CD86||PO3||FC|
|PE/Cyanine7 anti-mouse CD86||PO3||FC|
|Alexa Fluor® 700 anti-mouse CD86||PO3||FC|
|Brilliant Violet 421™ anti-mouse CD86||PO3||FC|
|Brilliant Violet 605™ anti-mouse CD86||PO3||FC|
|PerCP/Cyanine5.5 anti-mouse CD86||PO3||FC|
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