Brilliant Violet 605™ anti-mouse CD196 (CCR6) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

N-terminal peptide (aa. 2-38) of mouse CCR6 fused with GST

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Product Citations

1. Huang J, et al. 2021. Immunity. 54(4):673-686.e4. PubMed
2. Xu W et al. 2019. Immunity. 50(4):1054-1068 . PubMed
3. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
4. Hernández–Santos N, et al. 2018. Cell Host Microbe. 1.313194444. PubMed
5. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
6. Riding AM, et al. 2022. iScience. 25:104660. PubMed
7. Long C, et al. 2016. Toxicol Sci. 152: 85 - 98. PubMed
8. Li H, et al. 2021. Protein Cell. Online ahead of print. PubMed

RRID

AB_2562513 (BioLegend Cat. No. 129819)

Antigen Details

Structure

G-protein linked, seven transmembrane, domain-spanning chemokine receptor; 367 amino acids long and about 75% homology with human CCR6

Distribution

Expressed on spleen B cells, immature splenic dendritic cells in Peyer's patches, and some T cell subset

Function

Important in regulating mucosal immune response

Ligand/Receptor

CCL20/MIP-3a

Cell Type

B cells, Dendritic cells, T cells

Biology Area

Immunology

Molecular Family

CD Molecules, Cytokine/Chemokine Receptors, GPCR

Antigen References

1. Rossi D, et al. 1997. J. Immunol. 158:1033.
2. Lukacs N, et al. 2001. J. Exp. Med. 194:551.
3. Cook DN, et al. 2000. Immunity 12:495

Gene ID

12458 View all products for this Gene ID

UniProt

View information about CD196 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Other Formats

Certificate of Analysis