- B27 (See other available formats)
- Other Names
- Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. The B27 antibody reacts with the human interferon-γ. The B27 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.Product Details
- Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, African Green, Sooty Mangabey
- Antibody Type
- Host Species
- E. coli-expressed recombinant human IFN-γ
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 510™ and unconjugated antibody.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
- Excitation Laser
Violet Laser (405 nm)
- Application Notes
Flow Cytometry2: The fluorochrome-labeled B27 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Neutralization1,3,6,7: The Ultra-LEAF™ Purified antibody (Endotoxin <0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human IFN-γ bioactivity (Cat. No. 506531).
(PubMed link indicates BioLegend citation)
- Favre C, et al. 1989. Molec. Immunol. 26:17. (Neut)
- Kaur A, et al. 2002. J Virol. 76:3646.
- Abrams J, et al. 1992. Immunol. Rev. 127:5. (Neut)
- Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
- Rout N, et al. 2010. PLoS One 5:e9787. (FC)
- Acosta-Rodriguez EV, et al. 2007. Nat. Immunol. 9:942-9. (Neut)
- Gangur V, et al. 1998. FASEB J. 12:705-13. (Neut)
- Cytokine; dimer; 20-25 kD (Mammalian)
- Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
- Cell Sources
- CD8+ and CD4+ T cells, NK cells
- Cell Targets
- T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
- IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
- Cell Type
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Antigen References
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.
- Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
- Gene ID
- 3458 View all products for this Gene ID
- View information about IFN-gamma on UniProt.org
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