Brilliant Violet 421™ anti-RPS6 Phospho (Ser235/Ser236) Antibody

Pricing & Availability
Clone
A17020B (See other available formats)
Other Names
S6, 40S ribosomal protein S6
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A17020B_BV421_RPS6-Phospho_Antibody_081619
Human peripheral blood lymphocytes were stimulated with (filled histogram) or without (open histogram) Cell Activation Cocktail without Brefeldin A (Cat. No. 423302) for 15 minutes, fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401), and intracellularly stained with anti-RPS6 Phosopho (Ser235/Ser236) (clone A17020B) Brilliant Violet 421™.
  • A17020B_BV421_RPS6-Phospho_Antibody_081619
    Human peripheral blood lymphocytes were stimulated with (filled histogram) or without (open histogram) Cell Activation Cocktail without Brefeldin A (Cat. No. 423302) for 15 minutes, fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401), and intracellularly stained with anti-RPS6 Phosopho (Ser235/Ser236) (clone A17020B) Brilliant Violet 421™.
See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Avail. Save
608609 25 tests £111
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608610 100 tests £272
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Description

Ribosomal protein S6 (RPS6) is a key component of the small 40S ribosomal subunit and is the major substrate of protein kinases in eukaryotic ribosomes. In response to various cellular stimuli such as mitogenic stimulation, insulin, and increased nutrient availability, upstream kinases such as RSK and p70 kinases phosphorylate RPS6 at multiple serine sites. These modifications facilitate the recruitment of the  7-methylguanasine cap complex, thereby promoting the assembly of the translational pre-initiation complex and increased cellular protein synthesis capacity. RPS6 has been shown to be hyperphosphorylated in certain cancers, and phosphorylation is a critical determinant of pancreatic β–cell size and systemic glucose homeostasis function in diabeteic mouse models.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide from human RPS6 phosphorylated at Serines 235 and 236
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Due to complete conservation of the immunizing sequence between humans, mouse and rat, this clone is is predicted to react with rat RPS6 phosphorylated at serines 235 and 236.

RRID
AB_2814450 (BioLegend Cat. No. 608609)
AB_2814451 (BioLegend Cat. No. 608610)

Antigen Details

Structure
RPS6 is a 249 amino acid protein with a predicted molecular weight of 28.6 kD.
Distribution

Cytosol, nucleus, ER, ubiquitous expression.

Function
Phosphorylation events at a cluster of serine residues (Ser 235, 236, 240, and 244) at the carboxy-terminus of RPS6 results in increased global translational efficiency. These sites are dephosphorylated during growth arrest.
Interaction
RPS6AK1 and RPS6AK3; other subunits of 40S ribosomal complex.
Biology Area
Cell Biology, Cell Proliferation and Viability, Protein Synthesis, Signal Transduction
Molecular Family
Nuclear Markers, Phospho-Proteins
Antigen References
  1. Jefferies HB, et al. 1997. EMBO J. 16:3693.
  2. Ruvinsky I, et al. 2005. Genes. Dev.19:2199.
  3. Chumacher AM, et al. 2006. Biochemistry. 45:13614
  4. Roux PP, et al. 2007. J. Biol. Chem. 282:14056.
  5. Stevens C, et al. 2009. J. Biol. Chem. 284:334.
  6. Schlafli P, et al. 2011. FEBS J. 278:1757.
Gene ID
6194 View all products for this Gene ID
UniProt
View information about RPS6 Phospho on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 08/16/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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