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Brilliant Violet 421™ anti-mouse TIGIT (Vstm3) Antibody

Pricing & Availability
Clone
1G9 (See other available formats)
Regulatory Status
RUO
Other Names
VSIG9
Isotype
Mouse IgG1, κ
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Product Citations
publications
1G9_BV421_TIGIT_Antibody_1_052617
C57BL/6 mouse splenocytes were stimulated with anti-CD3 and anti-CD28 antibodies for 24 hours then stained with CD3 APC and TIGIT (clone 1G9) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • 1G9_BV421_TIGIT_Antibody_1_052617
    C57BL/6 mouse splenocytes were stimulated with anti-CD3 and anti-CD28 antibodies for 24 hours then stained with CD3 APC and TIGIT (clone 1G9) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • 1G9_BV421_TIGIT_Antibody_2_052617
Compare all formats See Brilliant Violet 421™ spectral data
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142111 50 µg £166
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Description

T cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as V-set and transmembrane domain-containing protein 3 (Vstm3), is a 26 kD, type I transmembrane protein and member of the CD28 family. TIGIT is expressed on activated T cells, follicular T helper, memory, and regulatory T cells as well as on NK cells. Its binding partners include CD155 (PVR) and CD112 (PVRL2). TIGIT is a negative regulator of NK and T cell activation. Engagement of TIGIT by dendritic cells results in their differentiation into a tolerogenic phenotype, with an increased secretion of IL-10 and a diminished production of IL-12. Mice deficient for TIGIT are more susceptible to autoimmune disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. 


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Joller N, et al. 2010. J. Immunol. 186:1338.
Product Citations
  1. Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed
  2. Markov SD, et al. 2021. Mol Cancer Ther. 20:2457. PubMed
  3. Marjanovic ND, et al. 2020. Cancer Cell. 38(2):229-246.e13. PubMed
  4. Wang S, et al. 2021. Parasite. 28:13. PubMed
RRID
AB_2687311 (BioLegend Cat. No. 142111)

Antigen Details

Structure
Type I transmembrane protein, 26 kD, member of the CD28 family. Contains 1 Ig-like domain and 1 cytoplasmic ITIM motif.
Distribution

Activated T cells, follicular T helper cells, NK, memory T cells, Tregs.

Function
Negative regulator of T cell activation and proliferation. Induction of tolerogenic dendritic cells.
Ligand/Receptor
CD155 (PVR) and CD112 (PVRL2).
Cell Type
Dendritic cells, NK cells, T cells, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
Adhesion Molecules, Immune Checkpoint Receptors
Antigen References

1. Levin SD, et al. 2011. Eur. J. Immunol. 41:902.
2. Yu X, et al. 2009. Nat. Immunol. 10:48.
3. Stanietsky N, et al. 2009. P. Natl. Acad. Sci. USA 106:17858.

Gene ID
100043314 View all products for this Gene ID
UniProt
View information about TIGIT on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 0    Revision Date: 05/30/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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