Brilliant Violet 421™ anti-mouse CD41 Antibody

Pricing & Availability
Clone
MWReg30 (See other available formats)
Other Names
Fibrinogen receptor, gpIIb/IIIa, integrin alpha IIb, CD41a
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
MWREG30_BV421_112811
C57BL/6 platelets were stained with CD41 (clone MWReg30) Brilliant Violet 421™ (filled histogram) or rat IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
  • MWREG30_BV421_112811
    C57BL/6 platelets were stained with CD41 (clone MWReg30) Brilliant Violet 421™ (filled histogram) or rat IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Avail. Save
133911 125 µL £127
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133912 50 µg £171
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Description

CD41, also known as integrin α2b and GPIIb, is a transmembrane glycoprotein that is expressed by platelets and megakaryocytes. It was reported that CD41 is also expressed on hematopoietic progenitors. CD41 associates with CD61 (integrin β3) to form complexes that interact with fibrinogen, fibronectin, von Willebrand factor, and thrombin. CD41 is required for platelet adhesion and aggregation. Defect of CD41 leads to disorders of coagulation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse platelets
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µl size: Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
µg size: 0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: depletion of platelets and functional assay in vivo.4,7 The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for in vivo studies (Cat. No. 133939).

Application References

(PubMed link indicates BioLegend citation)
  1. Nieswandt B, et al. 1999. Blood 94:684.
  2. Teeling JL, et al. 2001. Blood 98:1095.
  3. Bertrand JY, et al. 2005. P. Natl. Acad. Sci. USA 102:134.
  4. Nocito A, et al. 2007. Hepatology 45:369. (Deplete)
  5. Sullivan BP, et al. 2010. Toxicol. Sci. 115:286. (Deplete) PubMed
  6. van der Heyde HC, et al. 2005. Blood 105:1956. (FA)
  7. Marjon KD, et al. 2009. J. Immunol. 182:1397. (Deplete)
Product Citations
  1. Rao T, et al. 2015. Stem Cell Res. 14:307. PubMed
  2. Marks-Bluth J, et al. 2015. Mol Cell Biol. 35:2165. PubMed
  3. Souilhol C, et al. 2016. Nat Commun. 7:10784. PubMed
  4. Mizuno T, et al. 2017. Sci Rep. 7:42714. PubMed
  5. Yamamoto R et al. 2018. Cell stem cell. 22(4):600-607 . PubMed
  6. Commerford CD et al. 2018. Cell reports. . PubMed
RRID
AB_10960744 (BioLegend Cat. No. 133911)
AB_2650893 (BioLegend Cat. No. 133912)

Antigen Details

Structure
A transmembrane glycoprotein that is expressed by platelets and megakaryocytes
Distribution

Platelets, megakaryocytes, and hematopoietic progenitors

Function
Associate with CD61 (integrin β3) to form a complex which plays a major role in platelet adhesion and aggregation
Ligand/Receptor
Fibrinogen, fibronectin, von Willebrand factor, thrombin
Cell Type
Hematopoietic stem and progenitors, Megakaryocytes, Platelets
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Bakewell SJ, et al. 2003. P. Natl. Acad. Sci. USA 100:14205.
2. Phillips DR, et al. 1991. Cell. 65:359.

Gene ID
16399 View all products for this Gene ID
UniProt
View information about CD41 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 2    Revision Date: 03/19/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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