Biotinylated Recombinant Human PD-L2-Fc Chimera (carrier-free)

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Other Names
CD273, Programmed cell death 1 ligand 2 (PD-L2), B7 dendritic cell molecule, B7-DC, Butyrophilin B7-DC, PDCD1 Ligand 2, PDCD1L2, PDL2
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Biotinylated_Recom_Human_PD-L2-Fc_Chimera_CF_1_072219
When human PD-1-Fc Chimera is immobilized at 1.0 µg/mL, biotinylated human PD-L2-Fc Chimera binds with an EC50 of 3 - 12 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
  • Biotinylated_Recom_Human_PD-L2-Fc_Chimera_CF_1_072219
    When human PD-1-Fc Chimera is immobilized at 1.0 µg/mL, biotinylated human PD-L2-Fc Chimera binds with an EC50 of 3 - 12 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
  • Biotinylated_Recom_Human_PD-L2-Fc_Chimera_CF_2_072219
    Stability testing for Biotinylated Recombinant Human PD-L2-Fc Chimera. Biotinylated Recombinant Human PD-L2-Fc Chimera was aliquoted in PBS, pH 7.2, 5% glycerol at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4°C (Control). The samples were tested in a binding assay with human PD-1-Fc Chimera.
Cat # Size Price Quantity Avail. Save
785902 10 µg £84
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785904 25 µg £165
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785906 100 µg £491
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Description

PD-L2 is a type I transmembrane protein of 273 amino acids, and it is a member of the B7 family. Human PD-L2 has 70% amino acid identity to its mouse orthologue. PD-L2 is the second ligand for PD-1, and its binding to PD-1 leads to the inhibition of T cell receptor–mediated lymphocyte proliferation and cytokine secretion. PD-L2/PD-1 interaction suppresses immune responses against autoantigens and tumors and plays an important role in the maintenance of peripheral immune tolerance. Antibody blocking of PD-L2 on dendritic cells results in enhanced T cell proliferation and cytokine production. Interaction of PD-1 by PD-L1-Ig or PD-L2-Ig fusion protein has an inhibitory effect on proliferation and cytokine production during anti-CD3-mediated stimulation. PD-L2 is expressed in biopsies from human asthmatics and lungs of aeroallergen-treated mice and its expression correlates with the asthma severity. In vivo blockade of PD-L2 reduces the severity of allergen-driven airway hyperresponsiveness (AHR).  Blockage of PD-L2 induces the IL-12 production by DC; IL-12 may limit the severity of allergen-induced AHR antagonizing IL-13. In addition, blockade of PD-1 does not have an effect on AHR; therefore, PD-L2 controls asthma severity in a PD-1 independent manner. PD-L2 binds to a second receptor, RGMb (repulsive guidance molecule b) which is expressed in lung interstitial macrophages and alveolar epithelial cells and is a co-receptor for bone morphogenetic proteins BMP2/4. Blockade of the RGMb/PD-L2 interaction significantly impaired the development of respiratory tolerance since the initial T cell expansion is inhibited.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Source
Human PD-L2, amino acids Leu20-Pro219 (Accession # Q9BQ51) with C terminal human IgG1 Fc and 6His tag was expressed in 293E cells.
Molecular Mass
The unlabeled 445 amino acid recombinant protein has a predicted molecular mass of approximately 50.2 kD. The DTT-reduced and non-reduced protein migrate at approximately 70 kD and 140 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Leu.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2, 5% glycerol.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
10-25 µg sizes are bottled at 200 µg/mL. 100 µg size is lot-specific and bottled at the concentration indicated on the vial. (Please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 0.2 - 1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Activity
When human PD-1-Fc Chimera is immobilized at 1.0 µg/mL, biotinylated human PD-L2-Fc Chimera binds with an EC50 of 3 - 12 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Disulfide-linked homodimer, biotinylated via amines.
Function
Inhibits T cell mediated proliferation and cytokine production by CD4+ T cells. Regulates T cell responses. PD-L2 expression is upregulated on antigen-presenting cells by IFN-γ.
Interaction
T cell
Ligand/Receptor
PD-1
Bioactivity
Measured by its ability to bind human PD-1.
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules, Immune Checkpoint Receptors, Soluble Receptors
Antigen References
  1. Latchman Y, et al. 2001. Nat. Immunol. 2:261-68.
  2. Brown JA, et al. 2003. J. Immunol. 170:1257-266.
  3. Youngnak P, et al. 2003. Biochem. Biophys. Res. Commun. 307:672-77.
  4. Singh AK, et al. 2011. Allergy. 66:155-62.
  5. Lewkowich IP, et al. 2013. Mucosal Immunol. 10.1038/mi.2012.111.
  6. Xiao Y, et al. 2014. J. Exp. Med. 10.1084/jem.20130790.
  7. Bardhan K, et al. 2016. Front Immunol. 7:550.
Gene ID
80380 View all products for this Gene ID
UniProt
View information about PD-L2 on UniProt.org

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Go To Top Version: 1    Revision Date: 07/22/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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