Biotinylated Recombinant Human PD-1-Fc (CD279) Chimera (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
Programmed Cell Death Protein 1, PD1, PDCD1, CD279
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Product Citations
publications
Human_PD-1_BIOTIN_RECOM_CF_1_031023
Biotinylated recombinant human PD-1-Fc binds to immobilized human PD-L2 at 1 µg/mL (100 µL/well), with an EC50 of 3 - 12 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
  • Human_PD-1_BIOTIN_RECOM_CF_1_031023
    Biotinylated recombinant human PD-1-Fc binds to immobilized human PD-L2 at 1 µg/mL (100 µL/well), with an EC50 of 3 - 12 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
  • Human_PD-1_BIOTIN_RECOM_CF_2_031023
    Stability Testing for Biotinylated Recombinant Human PD-1-Fc. Biotinylated recombinant human PD-1-Fc was aliquoted in PBS, 5% glycerol at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested by their ability to bind to immobilized recombinant PD-L2 in a dose dependent manner. The ED50 for this effect is 3 -12 ng/mL.
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799504 25 µg £182
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799506 100 µg £462
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Description

Programmed death-1 (PD-1) is a type I transmembrane protein initially isolated from apoptosis-induced cells by subtractive hybridization. The amino acid sequence of mouse PD-1 shares 59.4% identity to the human counterpart, and a putative tyrosine kinase-association motif is well-conserved. Its extracellular region consists of a single Ig-like variable (IgV) domain. PD-1 is an inhibitory molecule expressed by activated B and T cells and has been implicated in immune tolerance. PD-L1 and PD-L2 are the ligands for PD-1 and their binding leads to the inhibition of T cell receptor-mediated lymphocyte proliferation and cytokine secretion. PD-1/PD-L1 interaction suppresses immune responses against autoantigens and tumors, and plays an important role in the maintenance of peripheral immune tolerance. Interaction between PD-1 and PD-L1 promote tolerance by blocking TCR-mediated signaling.  PD-L1 expression by tumor cells allow tumor progression by suppressing antitumor T-cell responses. Antibodies against PD-1 or PD-L1 lead to increased antitumor immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Biotinylated Human PD-1, amino acid Leu25-Gln167 (Accession # Q15116.3), with a linker (GS) and a C-terminal human IgG (Pro100-Lys330), followed by a Avi tag, was expressed in 293E cells. Human PD-1-Avi tag was site-specifically biotinylated by enzyme BirA.
Molecular Mass
The 391 amino acid recombinant protein has a predicted molecular mass of approximately 43.85 kD. The DTT-reduced and non-reduced protein migrates at approximately 62-70 kD and 120-130 KD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Leu.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, 5% glycerol.
Endotoxin Level
Less than 0.1 EU per µg cytokine as determined by the LAL method
Concentration
25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Biotinylated Human PD-1-Fc binds to immobilized human PD-L2 at 1 µg/mL (100 µL/well), with an EC50 of 3 -12 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Dimer
Distribution

Antigen-stimulated T and B cells, regulatory T cells, follicular T and B cells,  dendritic cells, and monocytes

Function
Inhibitory receptor on antigen-activated T cells that plays a critical role in induction and maintenance of immune tolerance
Interaction
APC, monocytes, dendritic cells, stromal cell, cancer cells
Ligand/Receptor
B7-H1 (PD-L1, CD274) and B7-DC (PD-L2, CD273)
Bioactivity
Measured by its ability to bind to human PD-L2-Fc
Cell Type
Lymphocytes, T cells
Biology Area
Adaptive Immunity, Cancer Biomarkers, Cell Biology, Immuno-Oncology, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References
  1. Ishida Y, et al. 1992. EMBO J. 11:3887-95.
  2. Fife BT, et al. 2009. Nature Immunol. 10:1185-92.
  3. Freeman GJ, et al. 2000. J Exp Med. 192:1027-34.
  4. Latchman Y, et al. 2001. Nat Immunol. 2:261-8.
  5. Iwai Y, et al. 2002. Proc Natl Acad Sci USA. 99:12293-7.
  6. Dai S, et al. 2014. Cell Immunol. 290:72-9.
  7. Tan S, et al. 2017. Nature Commun. 8:14369.
Gene ID
5133 View all products for this Gene ID
UniProt
View information about PD-1 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 03/10/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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