Biotin anti-rat IFN-γ Antibody

Pricing & Availability
Clone
Poly5188 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Isotype
Goat Polyclonal Ig
Ave. Rating
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Product Citations
publications
Poly5188_Biotin_IFNgamma_Antibody_1_ELISA_051316
  • Poly5188_Biotin_IFNgamma_Antibody_1_ELISA_051316
  • Poly5188_Biotin_IFNgamma_Antibody_2_WB_051316
    Western blot analysis of 50 ng recombinant human IFN-γ (lane 1), recombinant rat IFN-γ (lane 2), and recombinant mouse IFN-γ (lane 3) using biotin anti-rat IFN-γ (clone Poly5188) antibody followed by HRP Streptavidin secondary antibody.
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518803 50 µg £92.00
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Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Rat
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant rat IFN-γ
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by antigen affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested
WB - Verified

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.1 - 0.5 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of rat IFN-γ recombinant protein ranging from 2000 to 31 pg/mL are recommended for each ELISA plate. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection: The biotinylated poly5188 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified DB-1 antibody (Cat. No. 507801 or 507808) as the capture antibody and recombinant rat IFN-γ (Cat. No. 580009) as the standard.

Note: For testing rat IFN-γ in serum, plasma or cell culture supernatant, BioLegend's ELISA Legend Max™ Kits (Cat. No. 439007 & 439008) are specially developed and recommended.

Product Citations
  1. Allen KD, et al. 2020. Connect Tissue Res. 61:82. PubMed
RRID
AB_2561418 (BioLegend Cat. No. 518803)

Antigen Details

Structure
Cytokine, dimer, 40-80 kD (Mammalian)
Distribution
CD8+ and CD4+ T cells, NK cells
Function
Upregulated by IL-2, bFGF, EGF, downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
Interaction
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Ligand/Receptor
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
T cells, NK cells
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Gene ID
25712 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 3    Revision Date: 06/01/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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