Biotin anti-rat CD103 (αE Integrin) Antibody

Pricing & Availability
Clone
OX-62 (See other available formats)
Regulatory Status
RUO
Other Names
Integrin αE, integrin E, integrin αIEL, integrin αOX-62, Itgae
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
OX-62_Biotin_1_111710
LOU rat intestinal intraepithelial lymphocytes were stained with CD3 Alexa Fluor® 647 and biotinylated CD103 (clone OX-62, top) or mouse IgG1, κ isotype control (bottom), followed by SAV-PE.
  • OX-62_Biotin_1_111710
    LOU rat intestinal intraepithelial lymphocytes were stained with CD3 Alexa Fluor® 647 and biotinylated CD103 (clone OX-62, top) or mouse IgG1, κ isotype control (bottom), followed by SAV-PE.
  • OX-62_Biotin_2_111710
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205503 100 µg £89.00
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Description

CD103, also known as Integrin αE or integrin αIEL, is a single pass transmembrane protein composed by two chains, heavy and light, linked by a disulfide bond. It is expressed in the cell surface as an αE/β7 heterodimer. CD103 mediates adhesion of intraepithelial T cells (IEL) to the epithelium. CD103 is expressed by IEL, effector and memory Tregs, and dendritic cells and their immediate precursors. E-cadherin is the ligand for CD103.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Density gradient enriched lymph veiled cells from PVG rats
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen skin and intestine epithelium1,4, immunocytochemical staining of cytospin preparations4, and Western blot1.

Application References

(PubMed link indicates BioLegend citation)
  1. Brenan M, et al. 1992. J. Exp. Med. 175:1457. (IHC, WB)
  2. Chen-Woan M, et al. 1995. J. Immunol. Methods 178:157.
  3. Chen-Woan M, et al. 1996. J. Leuk. Biol. 59:196.
  4. Brenan M and Rees DJ. 2000. Eur. J. Immunol. 30:1527. (IHC)
RRID
AB_10683166 (BioLegend Cat. No. 205503)

Antigen Details

Structure
Single pass transmembrane protein, composed by two chains, heavy and light, linked by a disulfide bond. Associates with integrin β7 to form the αE/β7 complex.
Distribution

Intraepithelial T lymphocytes, effector and memory Tregs, dendritic cells and their immediate precursors.

Function
Mediates adhesion of intraepithelial T cells (IEL) to epithelium.
Ligand/Receptor
E-cadherin
Cell Type
Dendritic cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Varas A, et al. 1998. Blood 92:93.
2. Brissette-Storkus CS, et al. 2002. J. Leukoc. Biol. 71:941.
3. Hubert FX, et al. 2004. J. Immunol. 172:7485.
4. Tsuchihashi S, et al. 2006. J. Immunol. 176:616.
5. Lührmann A, et al. 2007. Immunol. Lett. 108:167.

Gene ID
83577 View all products for this Gene ID
UniProt
View information about CD103 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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