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Biotin anti-phycoerythrin (PE) Antibody

Pricing & Availability
Clone
PE001 (See other available formats)
Regulatory Status
RUO
Other Names
PE
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A_PE001_Biotin_111607
C57BL/6 thymocytes stained with RM4-5 PE only (top) or RM4-5 PE plus biotinylated PE001 (bottom), then detected with Sav-FITC
  • A_PE001_Biotin_111607
    C57BL/6 thymocytes stained with RM4-5 PE only (top) or RM4-5 PE plus biotinylated PE001 (bottom), then detected with Sav-FITC
  • B_PE001_Biotin_111607
    C57BL/6 thymocytes stained with RM4-5 PE only (top) or RM4-5 PE plus biotinylated PE001 (bottom), then detected with Sav-FITC
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Cat # Size Price Quantity Check Availability Save
408103 25 µg £37
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408104 100 µg £81
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Description

The PE001 antibody reacts with both phycoerythrin (PE) alone and PE conjugated with antibody or streptavidin, as well as PE tandem dyes such as PE/Cy5, PE/Cy5.5 and PE/Cy7. When PE001 antibody is used in a three-step staining procedure or separation of PE-antibody labeled cells, it does not quench PE fluorescence or react with tested mouse and human cells. The PE001 antibody can be used for separation of PE-antibody labeled cells or as a secondary step to amplify PE conjugated primary antibody signals (i.e. biotinylated PE001 as secondary step, followed by Streptavidin-PE).

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Phycoerythrin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Use at concentrations ≤ 0.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Taylor RT, et al. 2007. J. Immunol. 178:5659.
RRID
AB_2237499 (BioLegend Cat. No. 408103)
AB_2168923 (BioLegend Cat. No. 408104)

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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