Biotin anti-mouse/rat/human MIF Antibody

Pricing & Availability
Clone
Poly5240 (See other available formats)
Regulatory Status
RUO
Other Names
Macrophage migration inhibitory factor (MMIF), Glycosylation inhibiting factor (GIF), GLIF, Phenylpyruvate Tautomerase, L-Dopachrome Tautomerase, L-Dopachrome Isomerase
Isotype
Goat Polyclonal IgG
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Product Citations
publications
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524004 50 µg £154.00
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Description

MIF (human macrophage migration inhibitory factor) is a non-glycosylated polypeptide expressed by multiple cell types, which include activated T cells, macrophages, eosinophils, epithelial cells, and endothelial cells. It is secreted by response to diverse inflammatory stimuli, and has been associated with a clear pro-inflammatory and proatherogenic role in multiple studies involving patients and animals. MIF also positively correlates with obesity as well as insulin resistance and contributes to adipose tissue inflammation by modulating ATM functions. Furthermore, MIF has been described as a protein that plays an essential role in both innate and acquired immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Rat, Human
Antibody Type
Polyclonal
Host Species
Goat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA. For ELISA detection applications, a concentration of 0.1 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of MIF recombinant protein ranging from 10 to 0.16 ng/mL are recommended for each ELISA plate.

It is recommended that the reagent be titrated for optimal performance.

RRID
AB_2686961 (BioLegend Cat. No. 524004)

Antigen Details

Structure
115 amino acids with a predicted molecular weight of approximately 12.5 kD.Activated T-cells, macrophages, eosinophils, epithelial cells, and endothelial cells.
Distribution

Activated T-cells, macrophages, eosinophils, epithelial cells, and endothelial cells.

Function
MIF has been associated with a clear pro-inflammatory and proatherogenic role in cardiovascular diseases. MIF also plays important roles in adipose tissue inflammation and innate and acquired immunity.
Interaction
Monocytes and macrophages, mesenchymal cells, and B-cells.
Ligand/Receptor
CD44/CD74, CXCR4, CXCR2, and CXCR7.
Cell Type
T cells, Macrophages, Eosinophils, Epithelial cells, Endothelial cells
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Zhou H, et al. 2009. PLoS One. 4:e6087.
2. Potolicchio I, et al. 2003. J. Biol. Chem. 278:30889.
3. Meyer-Siegler K and Iczkowski K. 2005. BMC Cancer 5:73.
4. Babu SN, et al. 2012. Asian Pac. J. Cancer Prev. 13:1737.
5. van der Vorst EP, et al. 2015. Front Immunol. 6:373.
6. Kim BS, et al. 2015. Exp. Mol. Med. 47:e161.
7. Sanchez-Zamora YI, et al. 2014. J. Diabetes Res. 2014:804519.
8. Gilliver SC, et al. 2011. Exp. Dermatol. 20:1.
9. Bach JP, et al. 2008. Oncology 75:127.

Gene ID
17319 View all products for this Gene ID
UniProt
View information about MIF on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 0    Revision Date: 03/28/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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