Biotin anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody

Pricing & Availability
Clone
RB6-8C5 (See other available formats)
Other Names
Gr-1
Isotype
Rat IgG2b, κ
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RB6-8C5_Biotin_Ly-6GslashLy-6C_Antibody_1_FC_090116
C57BL/6 mouse bone marrow stained with biotinylated Ly-6G/Ly-6C (clone RB6-8C5, filled histogram) or biotinylated Rat IgG2b, κ isotype control (open histogram), followed by streptavidin PE.
  • RB6-8C5_Biotin_Ly-6GslashLy-6C_Antibody_1_FC_090116
    C57BL/6 mouse bone marrow stained with biotinylated Ly-6G/Ly-6C (clone RB6-8C5, filled histogram) or biotinylated Rat IgG2b, κ isotype control (open histogram), followed by streptavidin PE.
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108403 50 µg £27
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108404 500 µg £147
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Description

Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. In bone marrow, the expression levels of Gr-1 directly correlate with granulocyte differentiation and maturation; Gr-1 is also transiently expressed on bone marrow cells in the monocyte lineage. Immature Myeloid Gr-1+ cells play a role in the development of antitumor immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Raised against granulocytes of mouse origin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
IP, IHC, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C19. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A819. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.420.

The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity2, in vivo depletion3-5,9, immunoprecipitation1, immunohistochemical staining6 (including paraffin-embedded sections9,16,33-35, acetone-fixed frozen sections11 and zinc-fixed sections15), and Western blotting7. RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs)20.

Special Note: For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436).

Application References

(PubMed link indicates BioLegend citation)
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (IP)
  2. Brummer E, et al. 1984. J. Leukocyte Biol. 36:505. (CMCD)
  3. Stoppacciaro A, et al. 1993. J. Exp. Med. 178:151. (Deplete)
  4. Tumpey TM, et al. 1996. J. Virol. 70:898. (Deplete)
  5. Czuprynski CJ, et al. 1994. J. Immunol. 152:1836. (Deplete)
  6. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
  7. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (WB)
  8. Engwerda CR, et al. 2004. Am. J. Pathol. 165:2123.
  9. Brown CR, et al. 2004. Infect. Immun. 72:4956. (Deplete, IHC)
  10. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC) PubMed
  11. Li M, et al. 2006. P. Natl. Acad. Sci USA 103:11736. (IHC)
  12. Dzhagalov I, et al. 2007. Blood 109:1620. (FC) PubMed
  13. Fazilleau N, et al. 2007. Nature Immunol. 8:753. (FC) PubMed
  14. Heuser M, et al. 2007. Blood 110:1639. (FC) PubMed
  15. Wang T, et al. 2007. Infect. Immun. 75:1144. (IHC)
  16. Bosio CM, et al. 2007. J. Immunol. 178:4538. (IHC)
  17. Boehme SA, et al. 2009. Int. Immunol. 21:81. (IHC)
  18. Piao Y, et al. 2012. Neuro Oncol. 14:1379. PubMed
  19. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  20. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  21. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
  22. Fan Q, et al. 2014. Cancer Res. 74:471. PubMed
  23. Korrer MJ, et al. 2014. PLoS One. 9:91370. PubMed
  24. Morshed M, et al. 2014. J Immunol. 192:5314. PubMed
  25. Collins C, et al. 2014. PNAS. 111:9899. PubMed
  26. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
  27. Bianchi G, et al. 2014. Cell Death Dis. 5:1135. PubMed
  28. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  29. Roderick JE, et al. 2014. PNAS. 111:14436. PubMed
  30. Distel E, et al. 2014. Circ Res. 115:759. PubMed
  31. Iwai H, et al. 2015. Tuberculosis. 95:246. PubMed
  32. Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed
  33. Whiteland J, et al. 1994 J Histochem Cytochem 43:3 (IHC-P)
  34. Brown C, et al. 2003 J Immunology 171:2 (IHC-P)
  35. Obregon-Henao A, et al. PLoS One 8:11 (IHC-P)
Product Citations
  1. Ishidome T et al. 2017. EBioMedicine. 22:89-99 . PubMed
  2. Sevin M, et al. 2018. Nat Commun. 9:1431. PubMed
  3. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  4. Johnson JL et al. 2018. Immunity. 48(2):243-257 . PubMed
  5. Tanimura N et al. 2018. Developmental cell. 46(5):581-594 . PubMed
  6. Giambra V et al. 2018. Cell stem cell. 23(5):714-726 . PubMed
  7. Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed
  8. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  9. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  10. Luo W et al. 2018. Immunity. 48(2):313-326 . PubMed
  11. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  12. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  13. Parada-Kusz M, et al. 2018. Dis Model Mech. 11:. PubMed
  14. Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
  15. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  16. Moon H et al. 2017. Cell stem cell. 21(5):665-678 . PubMed
  17. Patel MM, et al. 2018. J Am Heart Assoc. 7:e010690. PubMed
  18. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  19. Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
  20. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  21. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  22. Ahmed AU, et al. 2017. J Immunol. 199(6):2128. PubMed
  23. Ito K et al. 2019. Cell Rep. 28(10):2480-2490 . PubMed
  24. Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed
  25. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  26. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  27. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
  28. Zhang C, et al. 2018. Cell Res. 28:323. PubMed
  29. Zhu C, et al. 2018. Sci Rep. 5.113194444. PubMed
  30. Davis FM, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2353. PubMed
  31. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  32. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  33. Mikami H, et al. 2020. Nat Commun. 11:1162. PubMed
  34. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  35. Shin JW, et al. 2020. Cell Death Dis. 0.667361111. PubMed
  36. He M, et al. 2020. Cell Metabolism. 31(3):580-591. PubMed
  37. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  38. Frohner IE, et al. 2020. Cell Rep. 30:3171. PubMed
  39. Andersen L, et al. 2020. Cell Reports. 29(13):4447-4459.e6.. PubMed
  40. Akk A, et al. 2019. Mol Immunol. 114:629. PubMed
  41. Getts D, et al. 2008. J Exp Med. 205:2319. PubMed
  42. Michel A, et al. 2013. J Immunol. 90:5534. PubMed
  43. Roderick J, et al. 2014. Proc Natl Acad Sci U S A. 111:14436. PubMed
  44. Matsuzaki Y, et al. 2015. Biomed Rep. 1: 91 - 97. PubMed
  45. Lutz J, et al. 2015. Nat Commun. 6: 8575. PubMed
  46. Kitano M, et al. 2016. Proc Natl Acad Sci U S A. 113: 1044 - 1049. PubMed
  47. Dubeykovskaya Z, et al. 2016. Nat Commun. 7:10517. PubMed
  48. Olsson A, et al. 2016. Nature. 10.1038/nature19348. PubMed
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  50. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
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  53. Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed
  54. Preglej T, et al. 2020. JCI Insight. 5(4):. PubMed
  55. Milner JJ, et al. 2020. Immunity. 52(5):808-824.e7. PubMed
  56. Gao Y, et al. 2020. Immunity. 52(6):1007-1021.e8. PubMed
  57. Lefkopoulos S, et al. 2020. Immunity. 53(5):934-951.e9. PubMed
  58. Olariu V, et al. 2021. Cell Reports. 34(2):108622. PubMed
  59. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
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RRID
AB_313368 (BioLegend Cat. No. 108403)
AB_313369 (BioLegend Cat. No. 108404)

Antigen Details

Structure
21-25 kD
Distribution

Granulocytes, monocytes

Cell Type
Granulocytes, Monocytes, Neutrophils
Biology Area
Immunology, Innate Immunity
Antigen References

1. Fleming TJ, et al. 1993. J. Immunol. 151:2399.
2. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
3. Goni O, et al. 2002. Int. Immunol. 14:1125.

Gene ID
17067 View all products for this Gene ID 546644 View all products for this Gene ID
UniProt
View information about Ly-6G/Ly-6C on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 3    Revision Date: 09/01/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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