Biotin anti-mouse IL-5 Antibody

Pricing & Availability
Clone
TRFK4 (See other available formats)
Other Names
Interleukin-5, Eosinophil differentiation factor (EDF), Eosinophil colony stimulating factor (Eo-CSF), B cell growth factor-2 (BCGF-2), B cell differentiation factor for IgM (BCDF-m), IgA enhancing factor (IgA-EF), T-cell replacing factor-1 (TRF-1)
Isotype
Rat IgG2a, κ
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
504401 50 µg £57
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504402 500 µg £155
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Description

IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. The native protein can have variable molecular weight as a result of glycosylation. Monomeric mouse IL-5 is a 113 amino acid protein with a reported molecular weight of 35-37 kD for the homodimeric protein. Mouse and human IL-5 are approximately 70% identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors and stimulates the activation, and differentiation of eosinophils, and promotes the generation of cytotoxic lymphocytes . Mouse IL-5 induces the differentiation and proliferation of pre-activated B-cells and stimulates the production and secretion of IgM and IgA by B-cells stimulated with bacterial endotoxin. The TRFK4 antibody reacts with mouse interleukin-5 (IL-5). The TRFK4 antibody can neutralize the bioactivity of natural or recombinant IL-5.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partially-purified T cell clone supernatant
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection, ELISPOT Detection

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25-1.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of mouse IL-5 recombinant protein ranging from 250 to 1 pg/ml are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes


ELISA1-4 or ELISPOT5-7 Detection: The biotinylated TRFK4 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified TRFK5 antibody (Cat. No. 504302/504308) as the capture antibody.
Additional reported applications (for the relevant formats) include: neutralization1 of mouse IL-5 bioactivity. The LEAF™ purified TRFK5 antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of mouse and human IL-5 bioactivity (Cat. No. 504308).
Note: For testing mouse IL-5 in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 431201 to 431206) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Schumacher, J., et al. 1988. J. Immunol. 141:1576.
  2. Abrams, J., et al. 1992. Immunol. Rev. 127:5.
  3. Abrams, J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
  4. Sander, B., et al. 1993. J. Immunol. Meth. 166:201.
  5. Taguchi, T., et al. 1990. J. Immunol. Meth. 128:65.
  6. Taguchi, T., et al. 1990. J. Immunol. 145:68.
  7. Klinman, D., et al. 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19.
RRID
AB_2296156 (BioLegend Cat. No. 504401)
AB_2127162 (BioLegend Cat. No. 504402)

Antigen Details

Structure
Cytokine; dimer; 40-50 kD (Mammalian)
Bioactivity
Growth/differentiation of B cells, eosinophils; proliferation/cell activation/differentiation of eosinophilic granulocytes
Cell Sources
Mast cells, T cells, eosinophils
Cell Targets
Eosinophils, B cells
Receptors
Heterodimer IL-5Rα (CD125); β-subunit (CDw131) in common with IL-3R, GM-CSFR
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Takatsu, K., et al. 1988. Immunol. Rev. 102:107.
3. Takatsu, K. 1992. Curr. Opin. Immunol. 4:299.
4. Takatsu, K. 1991. Microbiol. Immunol. 35:593.

Gene ID
16191 View all products for this Gene ID
UniProt
View information about IL-5 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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