Biotin anti-mouse IL-25 (IL-17E) Antibody

Pricing & Availability
Clone
M3704D11 (See other available formats)
Other Names
Interleukin 25, Interleukin 17E
Isotype
Rat IgG2a, κ
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538104 100 µg £151
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Description

IL-25 (also known as IL-17E) is a member of the IL-17 family, which includes IL-17A, IL-17B, IL-17C, IL-17F, and IL-17A/F. The primary sequence homology differs between family members, with mouse IL-17A and IL-17F having the highest homology (49%), while mouse IL-17A and IL-25 have the lowest (28%). IL-25 induces gene expression of IL-4, IL-5, and IL-13 in multiple tissues, and this induction results in T helper 2 (TH2)-type immune responses; this is characterized by an increase in serum IgE levels, pathologic changes in the lungs and digestive tract with eosinophilic infiltrates, an increase in mucus production, epithelial cell hyperplasia, and overall amplified allergic inflammation. IL-25 shares the receptor IL-17RB with IL-17B, although it binds with much higher affinity than IL-17B.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant Mouse IL-25
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA Detection applications, a concentration range of 1 - 8 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of IL-25 (IL-17E) recombinant protein ranging from 1600 to 25 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The biotinylated M3704D11 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the pure M3706F07 antibody (Cat. No. 676304) as the capture antibody and Recombinant Recombinant Mouse IL-25 (IL-17E) (ELISA Std.) (Cat. No. 579809).

Antigen Details

Structure
Heterodimer
Distribution

IL-25 is produced by mast cells, epithelial cells, alveolar macrophages, microglia, and Th2 cells

Function
IL-25 directly promotes Th2 cell differentiation and the expression of IL-4, IL-5, IL-13, CCL11 (eotaxin), and therefore, lung eosinophilia. IL-25 enhances IL-9 production by differentiated Th9 cells
Interaction
CD4+ NKT cells, macrophages, endothelial cells, eosinophils, and Th2 and Th9 cells
Ligand/Receptor
IL-25 binds to IL-17RB
Antigen References
  1. Hurst SD, et al. 2002. J. Immunol. 169:443.
  2. Ikeda K, et al. 2003. Blood 101:3594.
  3. Kang CM, et al. 2005. Am. J. Respir. Cell. Mol. Biol. 33:290.
  4. Kleinschek ME, et al. 2007. J. Exp. Med. 204:161.
  5. Terashima A, et al. 2008. J. Exp. Med. 205:2727.
  6. Angkasekwinai P, et al. 2010. Nat. Immunol. 11:250
Gene ID
140806 View all products for this Gene ID
UniProt
View information about IL-25 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 07/01/2019

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