- UH297 (See other available formats)
- Regulatory Status
- Other Names
- 1. Usui, M.
- Rat IgG1, κ
- Ave. Rating
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- Product Citations
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The UH297 antibody reacts specifically with mouse soluble immunoglobulin E of Igh-Ca haplotype (e.g., A, AKR, BALB/c, C3H/He, CBA). It does not react with IgE Igh-cb haplotype (e.g., C57BL/6, C57BL/10, SJL) and other Ig isotypes. UH297 mAb can be used for immunofluorescent staining of cell surface IgEa and ELISA applications.Product Details
- Antibody Type
- Host Species
- BALB/c mouse IgE anti-DNP mAb from DNPε 117 hybridoma
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
ELISA - Quality tested
FC, WB - Reported in the literature, not verified in house
- Recommended Usage
- Each lot of this antibody is quality control tested by ELISA. For ELISA detection, the suggested use of this reagent is 1-2 µg per ml. It's recommended that the reagent be titrated for optimal performance for each application.
(PubMed link indicates BioLegend citation)
- Usui M, et al. 1993. Immunogenetics. 37:301-304
- Yamada M, et al. 1994. Immunogenetics. 40:296-299
- Product Citations
AB_1937222 (BioLegend Cat. No. 408804)
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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