- MAR1-5A3 (See other available formats)
- Regulatory Status
- Other Names
- IFNAR1, IFN-αR1, IFN-α/β Receptor Subunit 1
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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IFNAR-1, the type I IFN receptor subunit 1, is coexpressed with IFNAR-2 on nearly all cells and make up the heterodimeric receptor complex that binds to all type I IFNs (IFN-α/β). Type I IFNs are a group of structurally and functionally related cytokines that have been shown to promote anti-viral, anti-microbial, anti-tumor, and autoimmune responses. Ligand binding to the IFN-α/β receptor complex leads to the tyrosine phosphorylation and activation of IFNAR-1-associated Tyk2 and IFNAR-2-associated Jak1 signal transductions.Product Details
- Antibody Type
- Host Species
- Exogenously expressed murine IFNAR1 extracellular domain
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
FC - Quality tested
IP, WB, ELISA - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, ELISA1, and blocking8,9. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 127322) (Endotoxin <0.01 EU/µg).
(PubMed link indicates BioLegend citation)
- Sheehan KC, et al. 2006. J. Interferon Cytokine Res. 26:804. (FC, Block, IP, WB, ELISA)
- Dunn GP, et al. 2005. Nat. Immunol. 6:722. (FC, WB)
- Miller JC, et al. 2008. J. Immunol. 181:8492. PubMed
- Habjan M, et al. 2009. J. Virol. 83:4365. PubMed
- Kelly-Scumpia KM, et al. 2010. J. Exp. Med. 207:319. PubMed
- Swanson CL, et al. 2010. J. Exp. Med. 207:1485. PubMed
- Marshall HD, et al. 2011. J Virol. epub. PubMed
- Swann JB, et al. 2007. J. Immunol. 178:7540. (Block)
- Jones GW, et al. 2011. FASEB J. 25:409. (Block)
- Mitzel DN, et al. 2014. J. Immunol. 192:4273. PubMed
- Product Citations
AB_1134250 (BioLegend Cat. No. 127305)
AB_1134249 (BioLegend Cat. No. 127306)
IFNAR1 and IFNAR2 are coexpressed on nearly all cells.
- Antigen References
1. Branca AA, et al. 1981. Nature 294:768.
2. Orchansky P, et al. 1984. J. Interferon Res. 4:275.
3. Hemmi S, et al. 1994. Cell 76:803.
4. Novick D, et al. 1994. Cell 77:391.
- Gene ID
- 15975 View all products for this Gene ID
- View information about IFNAR-1 on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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