Biotin anti-mouse/human NGF Antibody

Pricing & Availability
Clone
JKhNGF-2 (See other available formats)
Other Names
Nerve growth factor, NGF, β-NGF
Isotype
Mouse IgG1, κ
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publications
Cat # Size Price Quantity Avail. Save
509801 50 µg £67
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509802 500 µg £239
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Description

Nerve growth factor (NGF) is a small secreted protein that is important for the growth, maintenance, and survival of certain target neurons (nerve cells). NGF consists of three types of subunits, α, β and γ, which specifically interact to form a 7S, 130,000 molecular weight complex. This complex contains two identical 118-amino acid β-chains, which are solely responsible for nerve growth stimulating activity of NGF. NGF is essential for the development and phenotypic maintenance of neurons in the peripheral nervous system (PNS) and for the functional integrity of cholinergic neurons in the central nervous system (CNS).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Human, 2.5S, 7S, and β-NGF forms Cross-Reactivity*: Cattle (Bovine, Cow), Viper
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
recombinant human NGF (c-terminus)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 1 - 3 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. Additionally, BioLegend recommends the usage of Tris-Buffered Saline (TBS), pH = 7.2 for all washes and incubations, except substrate buffer. Do not use PBS as results may vary dramatically.

Application Notes

ELISA Detection (mouse): The biotinylated JKhNGF-2 antibody is useful as the detecting antibody in a sandwich ELISA assay, when used in conjunction with the purified JKmNGF-1 antibody (Cat. No. 509702) as the capture antibody for detecting mouse NGF.
ELISA Detection (human): The biotinylated JKhNGF-2 antibody is useful as the detecting antibody in a sandwich ELISA assay, when used in conjunction with the purified JKhNGF-1 antibody (Cat. No. 509602) as the capture antibody for detecting human NGF.

RRID
AB_2152401 (BioLegend Cat. No. 509801)
AB_2152400 (BioLegend Cat. No. 509802)

Antigen Details

Structure
Kallikrein family of serine proteases; dimer; 13.5 kD (Mammalian)
Bioactivity
Enhances survival, growth, neurotransmitter biosynthesis of sympathetic, sensory neurons; neurotrophic factor; cutaneous innervation; growth, differentiation, survival B lymphocytes; possible role in allergy, tissue repair
Cell Sources
Hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells, eosinophils
Cell Targets
Neurons, B cells, mast cells, eosinophils
Receptors
Low-affinity NGF receptor (LNGFR, p75 LNGFR, fast NGF receptor); high-affinity NGF receptor (trkA)
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Levi A, et al. 1991. Annu. Rev. Pharmacol. Toxicol. 31:205.
3. Varon S, et al. 1991. Adv. Exp. Med. Biol. 296:267.
4. Micera A, et al. 2003. Cytokine Growth Factor Rev. 14:369.

Regulation
Upregulated by glutamate, vitamin D3, IL-6, FGF-basic, astrocyte-specific IL-1, TNF-α, PDGF, TGF-β; downregulated by GABAergic neuronal activity, glucocorticoids, Schwann cell-specific TGF-β
Gene ID
18049 View all products for this Gene ID 4803 View all products for this Gene ID
UniProt
View information about NGF on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 07/22/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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