Biotin anti-mouse CD90.2 (Thy1.2) Antibody

Pricing & Availability
Clone
53-2.1 (See other available formats)
Regulatory Status
RUO
Other Names
Thy-1.2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
53-2.1_Biotin_081910
C57BL/6 mouse splenocytes stained with biotinylated CD90.2 (53-2.1), followed by Sav-PE (the FcR- mediated binding was blocked by pre-incubation with anti-mouse CD16/32, clone 93)
  • 53-2.1_Biotin_081910
    C57BL/6 mouse splenocytes stained with biotinylated CD90.2 (53-2.1), followed by Sav-PE (the FcR- mediated binding was blocked by pre-incubation with anti-mouse CD16/32, clone 93)
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140313 50 µg £34.00
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140314 500 µg £127.00
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Description

CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy-1.2, a GPI-linked membrane molecule. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining1 of frozen tissue section, immunofluorescence2, and immunoprecipitation3. Does not react with Thy-1.1 (CD90.1).

Application References

(PubMed link indicates BioLegend citation)
  1. Aldrich M, et al. 2003. J. Immunol. 171:5562. (IHC)
  2. Jameson J, et al. 2004. J. Immunol. 172:3573. (IF)
  3. Okada C, et al. 1990. J. Immunol. 144:3473. (IP)
Product Citations
  1. Schwartz DM et al. 2019. Immunity. 50(1):106-120 . PubMed
  2. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  3. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
RRID
AB_10640826 (BioLegend Cat. No. 140313)
AB_10643274 (BioLegend Cat. No. 140314)

Antigen Details

Structure
Ig superfamily, 25-35 kD
Distribution

Hematopoietic stem cells and neurons, epithelial cells, fibroblasts, neurons, all thymocytes, and peripheral T cells of Thy-1.2 bearing mice

Function
Lymphocyte costimulation, proliferation, and differentiation of hematopoietic stem cells
Ligand/Receptor
CD45
Cell Type
Epithelial cells, Fibroblasts, Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
Molecular Family
CD Molecules
Antigen References

1. Borrello M, et al. 1996. Cell. Immunol.173:198.
2. Radrizzani M, et al. 1995. J. Neurosci. Res. 42:220.
3. Williams A, et al. 1982. Science 216:696.

Gene ID
21838 View all products for this Gene ID
UniProt
View information about CD90.2 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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