- TR75-32.4 (See other available formats)
- Other Names
- TNFRII, TNFR type II, p75, TNFR80, TNFRSF1B
- Armenian Hamster IgG
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- Product Citations
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CD120b is a 75 kD type I transmembrane protein, also known as Tumor Necrosis Factor Receptor Type II (TNFRII) or p75. It is expressed on a variety of cells at low levels; the expression is upregulated upon activation. This receptor binds both TNF-α and LT-α (also known as TNF-β). In association with TRAF1 and TRAF2, the receptor crosslinking induced by TNF-α or LT-α trimers is critical for signal transduction, leading to apoptosis, NF-kB activation, increased expression of proinflammatory genes, tumor necrosis, and cell differentiation depending on cell type and differentiation state. The TR75-32.4 antibody has been shown to block ligand-induced receptor signaling.Product Details
- Antibody Type
- Host Species
- Armenian Hamster
- E. coli -expressed mouse Type II TNFR
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
FC - Quality tested
ELISA Detection - Reported in the literature
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay and/or immunofluorescent staining with flow cytometric analysis. For ELISA detection applications, a concentration range of 0.125-0.5 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of TNF R Type II/p75 recombinant protein ranging from 250 to 4 pg/ml are recommended for each ELISA plate. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Flow Cytometry: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 113204) or biotinylated anti-Armenian hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)).
ELISA Detection1: The biotinylated TR75-32.4 antibody is useful as a detection antibody when paired with the purified TR75-54.7 antibody (Cat. No. 113303/113304) as the capture antibody.
Additional reported applications (for the relevant formats) include: immunofluorescent staining, immunoprecipitation1 and receptor blocking1. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 113202).
(PubMed link indicates BioLegend citation)
- Sheehan KC, et al. 1995. J. Exp. Med. 181:607.
AB_313538 (BioLegend Cat. No. 113203)
- TNFR superfamily, 75 kD
- Variety of cell types at low levels
- Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation
- Ligand Receptor
- TNF-α, LT-α (TNF-β)
- Cell Type
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
1. Aggarwal BB, et al. 1985. Nature 318 665.
2. Chan FKM, et al. 2000. Science 288:2351.
3. Loetscher H, et al. 1990. Cell 61:351.
4. Rothe J, et al. 1993. Nature 364:798.
- Gene ID
- 21938 View all products for this Gene ID
- View information about CD120b on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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