- BL23 (See other available formats)
- Other Names
- IL-17, IL-17AF, IL-17A/F, Interleukin 17A, Interleukin 17AF, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
- Mouse IgG1, κ
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- Product Citations
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IL-17 or IL-17A originally identified as cytotoxic T lymphocyte-associated antigen-8 (CTLA-8) in mouse, is a founding member of the IL-17 family of cytokines. IL-17A is mainly produced by a small subset of T helper cells called Th17 cells. IL-17A is a 155 amino acid protein that is a disulfide linked, homodimeric, secreted glycoprotein with a molecular mass of 35 kD. The amino acid sequence of human IL-17A is 63% and 58% identical to mouse and rat IL-17A, respectively, and 72% identical to the ORF13 of Herpesvirus saimiri. IL-17A exhibits multiple biological activities including the induction of various proinflammatory cytokines and chemokines such as IL-6, IL-8, G-CSF, GM-CSF, GRO-α, and MCP-1 on a variety of cell types. IL-17A is also involved in the activation of NF-kB and costimulation of T cell proliferation. As a result of these roles, IL-17A has been linked to many immune/autoimmune related diseases including rheumatoid arthritis, asthma, lupus, and multiple sclerosis.
The IL-17A/F heterodimer consists of the monomeric units of IL-17A and IL-17F, which share 50% amino acid sequence identity. It has been shown to be a naturally expressed and biologically active molecule. While much has been studied about IL-17A and IL-17F, it is still unclear how each distinctively induces the inflammatory response, and what role IL-17A/F also plays in it. It is known that all three cytokines are produced in different amounts, and each has different degrees of pro-inflammatory effects. All seem to interact with the IL-17RA/IL-17RC receptor complex, and signal via the NF-κB pathway to induce secretion of inflammatory cytokines and recruit granulocytes.
BioLegend's purified BL23 antibody is IL-17A specific and can be paired with biotinylated Poly5189 to recognize IL-17A or with purified anti-human IL-17F clone Poly5166 to recognize the IL-17A/F heterodimer.
- Antibody Type
- Host Species
- Recombinant protein
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ELISA Detection - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA Detection applications, a concentration range of 0.4 - 3 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of IL-17A/F recombinant protein ranging from 10.94 to 700 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The biotinylated BL23 antibody (Cat. No. 512705) is is anti-human IL-17A and useful as the detection antibody in a sandwich ELISA assay. When used in conjunction with the pure Poly5166 antibody (Cat. No. 516601), it can recognize the IL-17A/F heterodimer (Cat. No. 580609). The pure BL23 antibody (Cat. No. 512702) can pair with biotinylated Poly5189 to recognize IL-17A.
AB_2783336 (BioLegend Cat. No. 512705)
- Cytokine; dimer
IL-17A is largely produced by activated memory T lymphocytes, CD4+ T helper cells (Th17), neutrophils, CD8(+), NK, and gamma-delta T cells (3).
- IL-17A is a potent regulator of granulopoiesis and neutrophil recruitment under normal and inflammatory conditions. Organ overexpression of IL-17A increases circulating neutrophil numbers and recruitment into the organs by induction of CXCL2, IL-1β, and G-CSF. Il-17A induces CXCL1, CXCL2, CXCL5, and CXCL8 in human epithelial cells. IL-17A also cooperates with TLR ligands, IL-1 beta, and TNF alpha to enhance inflammatory reactions and stimulate production of beta-defensins and other antimicrobial peptides (1, 2).
- IL-17AR is expressed in epithelial cells, fibroblasts, B and T lymphocytes, myelomonocytic cells, marrow stromal cells, synovial endothelial cells and chondrocytes from arthritic patients express IL-17R (4)
- Ligand Receptor
- IL-17A signals through a heteromeric receptor composed of IL-17RA and IL-17RC.
- Cell Type
- Neutrophils, NK cells, T cells
- Antigen References
- Yu JJ and Gaffen SL. 2008. Front Biosci 13:170.
- Toy D, et al. 2006. J. Immunol. 177:36.
- Benghiat FS, et al. 2009. Transplant Rev 23:11.
- Honorati MC, et al. 2001. Rheumatology 40:522.
- Rouvier E, et al. 1993 J. Immunol. 150:5445.
- Liang SC, et al. 2007. J. Immunol. 179:7791.
- Ouyang W, et al. 2008. Immunity 28:454.
- Gene ID
- 3605 View all products for this Gene ID
- View information about IL-17A on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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