Biotin anti-human IL-17A Antibody

Pricing & Availability
Clone
BL23 (See other available formats)
Other Names
IL-17, IL-17AF, IL-17A/F, Interleukin 17A, Interleukin 17AF, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
Isotype
Mouse IgG1, κ
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512705 100 µg £151
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Description

IL-17 or IL-17A originally identified as cytotoxic T lymphocyte-associated antigen-8 (CTLA-8) in mouse, is a founding member of the IL-17 family of cytokines. IL-17A is mainly produced by a small subset of T helper cells called Th17 cells. IL-17A is a 155 amino acid protein that is a disulfide linked, homodimeric, secreted glycoprotein with a molecular mass of 35 kD. The amino acid sequence of human IL-17A is 63% and 58% identical to mouse and rat IL-17A, respectively, and 72% identical to the ORF13 of Herpesvirus saimiri. IL-17A exhibits multiple biological activities including the induction of various proinflammatory cytokines and chemokines such as IL-6, IL-8, G-CSF, GM-CSF, GRO-α, and MCP-1 on a variety of cell types. IL-17A is also involved in the activation of NF-kB and costimulation of T cell proliferation. As a result of these roles, IL-17A has been linked to many immune/autoimmune related diseases including rheumatoid arthritis, asthma, lupus, and multiple sclerosis.

The IL-17A/F heterodimer consists of the monomeric units of IL-17A and IL-17F, which share 50% amino acid sequence identity. It has been shown to be a naturally expressed and biologically active molecule. While much has been studied about IL-17A and IL-17F, it is still unclear how each distinctively induces the inflammatory response, and what role IL-17A/F also plays in it. It is known that all three cytokines are produced in different amounts, and each has different degrees of pro-inflammatory effects. All seem to interact with the IL-17RA/IL-17RC receptor complex, and signal via the NF-κB pathway to induce secretion of inflammatory cytokines and recruit granulocytes.

BioLegend's purified BL23 antibody is IL-17A specific and can be paired with biotinylated Poly5189 to recognize IL-17A or with purified anti-human IL-17F clone Poly5166 to recognize the IL-17A/F heterodimer.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA Detection applications, a concentration range of 0.4 - 3 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of IL-17A/F recombinant protein ranging from 10.94 to 700 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The biotinylated BL23 antibody (Cat. No. 512705) is is anti-human IL-17A and useful as the detection antibody in a sandwich ELISA assay.  When used in conjunction with the pure Poly5166 antibody (Cat. No. 516601), it can recognize the IL-17A/F heterodimer (Cat. No. 580609).  The pure BL23 antibody (Cat. No. 512702) can pair with biotinylated Poly5189 to recognize IL-17A.

RRID
AB_2783336 (BioLegend Cat. No. 512705)

Antigen Details

Structure
Cytokine; dimer
Distribution

IL-17A is largely produced by activated memory T lymphocytes, CD4+ T helper cells (Th17), neutrophils, CD8(+), NK, and gamma-delta T cells (3).

Function
IL-17A is a potent regulator of granulopoiesis and neutrophil recruitment under normal and inflammatory conditions. Organ overexpression of IL-17A increases circulating neutrophil numbers and recruitment into the organs by induction of CXCL2, IL-1β, and G-CSF. Il-17A induces CXCL1, CXCL2, CXCL5, and CXCL8 in human epithelial cells. IL-17A also cooperates with TLR ligands, IL-1 beta, and TNF alpha to enhance inflammatory reactions and stimulate production of beta-defensins and other antimicrobial peptides (1, 2).
Interaction
IL-17AR is expressed in epithelial cells, fibroblasts, B and T lymphocytes, myelomonocytic cells, marrow stromal cells, synovial endothelial cells and chondrocytes from arthritic patients express IL-17R (4)
Ligand Receptor
IL-17A signals through a heteromeric receptor composed of IL-17RA and IL-17RC.
Cell Type
Neutrophils, NK cells, T cells
Antigen References
  1. Yu JJ and Gaffen SL. 2008. Front Biosci 13:170.
  2. Toy D, et al. 2006. J. Immunol. 177:36.
  3. Benghiat FS, et al. 2009. Transplant Rev 23:11.
  4. Honorati MC, et al. 2001. Rheumatology 40:522.
  5. Rouvier E, et al. 1993 J. Immunol. 150:5445.
  6. Liang SC, et al. 2007. J. Immunol. 179:7791.
  7. Ouyang W, et al. 2008. Immunity 28:454.
Gene ID
3605 View all products for this Gene ID
UniProt
View information about IL-17A on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 12/28/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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