Biotin anti-human IFN-α2 Antibody

Pricing & Availability
Clone
BL25372 (See other available formats)
Other Names
IFN-alpha 2B, IFN-alphaA, IFNA2, IFNA2B, Interferon Alpha 2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
BL25372_Biotin_IFN-a2_Antibody_122618
Coating Buffer B, M4509E12 at 4 µg/mL as capture antibody, Blocked Assay Diluent A (ADA) 1 hr, lyophilized standard 2 hr in Assay Buffer G 50:50 with Matrix A run 12.5 – 800 pg/mL, BL25372 (MT-2) at 0.1 µg/mL 1 hr for detection in ADA, 0.8 µg/mL Avidin HRP in ADA 30 min, TMB F 15 min (in house 76335).
  • BL25372_Biotin_IFN-a2_Antibody_122618
    Coating Buffer B, M4509E12 at 4 µg/mL as capture antibody, Blocked Assay Diluent A (ADA) 1 hr, lyophilized standard 2 hr in Assay Buffer G 50:50 with Matrix A run 12.5 – 800 pg/mL, BL25372 (MT-2) at 0.1 µg/mL 1 hr for detection in ADA, 0.8 µg/mL Avidin HRP in ADA 30 min, TMB F 15 min (in house 76335).
Cat # Size Price Quantity Avail. Save
537204 100 µg £151
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Description

Interferons are divided into type I, II, and III. Type I IFNs (IFN-α and IFN-β) are most abundant in number, distribution, and expression. Also, they are highly conserved among mammals in both structure and function. IFN-α2 has been used in the treatment of cancer such as bladder cancer, hepatocellular carcinoma, and leukemia. IFN-α2 augments the suppressed immune functions in patients with head and neck squamous cell carcinoma (HNSCC). IFN-α2 initiated T and NK cell mediated cytotoxicity of tumor cells through IFNγ dependent and independent mechanisms. IFN-α2 enhances suppressed T cell cytotoxicity by stimulation of the perforin-granzyme B system (IFNγ dependent). Also, IFN-α2 induces the expression of perforin-granzyme B in NK cells (NK mediated cytotoxicity, IFNγ independent). In a preliminary study, IFN-α2 appears to be an effective immunostimulator and impacts the clinical outcome in tongue squamous cell carcinoma patients. IFN-α had been used in the treatment of chronic hepatitis C (CHC); nevertheless, IFN-α is relatively unstable and requires frequent parenteral administration. Pegylation of IFN-α, polyethylene glycol (PEG)-IFN-α, reduces in vitro activity but increase the stability and plasma half-life of IFN-α; therefore, PEG-IFN-α has replaced IFN-α in CHC treatment.     

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA Detection applications, a concentration range of 0.05 - 0.4 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of IFN-α2 recombinant protein ranging from 12.5 to 800 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The biotinylated BL25372 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the pure M4509E12 antibody (Cat. No. 537102) as the capture antibody and Recombinant Human IFN-α2 (ELISA Std.) (Cat. No. 592709)

RRID
AB_2783347 (BioLegend Cat. No. 537204)

Antigen Details

Distribution

IFN-α is ubiquitously expressed.

Function
IFN-α stimulates cytotoxic T lymphocytes and natural killer cell function. IFN-α has immunomodulatory and antiviral properties. IFNα2a induces CXCL10 in human microvascular endothelial cells and pulmonary artery smooth muscle cells. It is induced by viral infection.
Interaction
A variety of cells express the IFNα receptor (IFNAR).
Ligand Receptor
IFNAR complex has two components, IFNAR1 and IFNAR2.
Antigen References
  1. Bose A and Baral R. 2007. Immunol Lett. 108:68.
  2. Zhao W, et al. 2008. J Immunol. 180:5483.
  3. Badiger R, et al. 2012. Plos One. 7:e46779.
  4. Aghemo A, et al. 2010. Nat Rev Gastroenterol Hepatol. 7:485.
  5. Becker-Merok A, et al. 2013. Lupus. 22:155.
  6. Mukherjee KK, et al. 2012. Indian J Med Res. 136:54.
Gene ID
3440 View all products for this Gene ID
UniProt
View information about IFN-α2 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 12/26/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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