Biotin anti-human CD279 (PD-1) Antibody

Pricing & Availability
Clone
NAT105 (See other available formats)
Regulatory Status
RUO
Other Names
PD1
Isotype
Mouse IgG1, κ
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Product Citations
publications
NAT105_biotin_CD279_Antibody_1_112217
Human peripheral blood lymphocytes were stained with CD3 APC and CD279 (clone NAT105) Biotin (top), or mouse IgG1, κ Biotin isotype control (bottom).
  • NAT105_biotin_CD279_Antibody_1_112217
    Human peripheral blood lymphocytes were stained with CD3 APC and CD279 (clone NAT105) Biotin (top), or mouse IgG1, κ Biotin isotype control (bottom).
  • NAT105_biotin_CD279_Antibody_2_112217
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367418 100 µg £185.00
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Description

Programmed cell death 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune diseases. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1, CD274) and PD-L2 (B7-DC, CD273) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. The interaction with CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
YT cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats of this clone) include: immunofluorescence staining.

We recommend using our Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB) (Cat. No. 929501).

Application References

(PubMed link indicates BioLegend citation)
  1. Roncador G, et al. 2007. Haematologica 92:1059.
  2. Nam-Cha SH, et al. 2008. Am. J. Surg. Pathol. 32:1252.
  3. Rodriguez-Pinilla SM, et al. 2009. Am. J. Surg. Pathol. 33:81.
RRID
AB_2721496 (BioLegend Cat. No. 367418)

Antigen Details

Structure
55 kD type I transmembrane protein.
Distribution

Transiently expressed on CD4- and CD8- thymocytes, upregulated in thymocytes and splenic T and B lymphocytes, and is expressed on activated myeloid cells.

Function
Signaling, co-stimulation (co-inhibition), immunoglobulin superfamily.
Interaction
SHP-1 and SHP-2.
Ligand/Receptor
PD-L1 (CD274) and PD-L2 (CD273).
Cell Type
Thymocytes, T cells, B cells, Tregs
Biology Area
Immunology, Cancer Biomarkers, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Francisco LM, Sage PT, and Sharpe AH. 2010. Immunological Rev. 236:219.

Gene ID
5133 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 0    Revision Date: 11/22/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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