Biotin anti-human CD22 Antibody

Pricing & Availability
Clone
S-HCL-1 (See other available formats)
Other Names
BL-CAM, Siglec-2, Lyb8
Isotype
Mouse IgG2b, κ
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Product Citations
publications
S-HCL-1_Biotin_CD22_Antibody_FC_1_112414
Human peripheral blood lymphocytes were stained with CD19 Alexa Fluor® 647 and biotinylated CD22 clone S-HCL-1 (top) or biotinylated mouse IgG2b, κ isotype control (bottom), followed by SAV-PE.
  • S-HCL-1_Biotin_CD22_Antibody_FC_1_112414
    Human peripheral blood lymphocytes were stained with CD19 Alexa Fluor® 647 and biotinylated CD22 clone S-HCL-1 (top) or biotinylated mouse IgG2b, κ isotype control (bottom), followed by SAV-PE.
  • S-HCL-1_Biotin_CD22_Antibody_FC_2_112414
Cat # Size Price Quantity Avail. Save
363510 100 µg £106
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Description

CD22 is a 130 kD type I transmembrane glycoprotein also known as Siglec-2 and BL-CAM and is a member of the immunoglobulin superfamily (sialoadhesion subgroup). CD22 is expressed in the cytoplasm of pro-B and pre-B cells, and on the surface of mature B and activated B cells, but not on plasma cells. CD22 is present in the B cell receptor complex and associates with SHP-1, Syk, Lck, Lyn, and phospholipase Cγ1. A primary function of CD22 is thought to be in limiting antigen receptor signaling by modulating B cell activation threshold. CD22 has been shown to bind to CD45RO and CD75, although the natural ligands for this molecule remain controversial.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes
  1. Nitschke L. 2005. Curr. Opin. Immunol. 17:290
  2. Foon Ka, et al. 1986. Blood. 68:297
  3. Schwarting R, et al. 1985. Blood. 65:974
  4. Campana D, et al. 1985. J. Immunol. 134:1524
RRID
AB_2564615 (BioLegend Cat. No. 363510)

Antigen Details

Structure
Ig superfamily, a type I glycosylated integral transmembrane protein, 120-130 kD
Distribution

B cells

Function
Adhesion, B-monocyte and B-T interactions
Ligand/Receptor
CD45RO, CD75
Cell Type
B cells
Biology Area
Immunology
Molecular Family
CD Molecules, Siglec Molecules
Antigen References
  1. Clark E. 1993. J. Immunol. 150:4715.
  2. Shan D, et al. 1995. J. Immunol. 154:4466.
Gene ID
933 View all products for this Gene ID
UniProt
View information about CD22 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 12/01/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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