Biotin anti-human CD146 Antibody

Pricing & Availability
Clone
P1H12 (See other available formats)
Workshop
HCDM listed
Other Names
Muc-18, MCAM, Mel-CAM, S-endo
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P1H12_Biotin_CD146_Antibody_072619.png
Human cervical cancer cell line, HeLa, was stained with CD146 (clone P1H12) biotin (filled histogram) or mouse IgG1, κ biotin isotype control (open histogram), followed by Streptavidin-PE.
  • P1H12_Biotin_CD146_Antibody_072619.png
    Human cervical cancer cell line, HeLa, was stained with CD146 (clone P1H12) biotin (filled histogram) or mouse IgG1, κ biotin isotype control (open histogram), followed by Streptavidin-PE.
Cat # Size Price Quantity Avail. Save
361036 100 µg £198
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Description

CD146 is a 118 kD integral transmembrane glycoprotein that is also known as MUC18, S-Endo, MCAM, and Mel-CAM (melanoma cell adhesion molecule). It belongs to the immunoglobulin superfamily. CD146 is expressed on melanoma cells, epithelial cells, endothelial cells, fibroblasts, activated T cells, multipotent mesenchymal stromal cells, and activated keratinocytes. CD146 mediates heterophilic cell adhesion and regulates monocyte transendothelial migration. The ligand of CD146 remains to be identified.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Cross-Reactivity: Mouse, Canine2, 5, Rabbit
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Cultured human umbilical cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Application Notes

Additional reported applications (for the relevant formats of this clone) include: Western blotting3 and IHC1,5

Application References

(PubMed link indicates BioLegend citation)
  1. Solovey A, et al. 1997. N. Engl. J. Med. 337:1584. (FC, IHC)
  2. Lamerato-Kozicki AR, et al. 2006. Exp. Hematol. 34:870. (FC)
  3. Balint K, et al. 2005. J. Clin. Invest. 115:3166. (WB)
  4. Neskey DM, et al. 2008. J. Exp. Clin. Cancer Res. 27:61. (ELISA)
  5. Kamstock D, et al. 2006. Cancer Gene Therap. 13:306. (IHC)

Antigen Details

Structure
118 kD integral glycoprotein, Ig superfamily
Distribution

Melanoma cells, epithelial cells, endothelial cells, activated T cells, multipotent mesenchymal stromal cells

Function
Adhesion, monocytes transendothelial migration
Cell Type
Endothelial cells, Epithelial cells, Mesenchymal cells, Mesenchymal Stem Cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Pickl WF, et al. 1997. J. Immunol. 158:2107.
2. Weninger W, et al. 2000. J. Invest. Dermatol. 115:219.
3. Sorrentino A, et al. 2008. Exp. Hematol. 36:1035.
4. Bardin N, et al. 2009. Arterioscler. Thromb. Vasc. Biol. 29:746.

Gene ID
4162 View all products for this Gene ID
UniProt
View information about CD146 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 07/26/2019

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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