Biotin anti-human CCL20 (MIP-3α) Antibody

Pricing & Availability
Clone
L2418G3 (See other available formats)
Regulatory Status
RUO
Other Names
CCL20, MIP-3ž€, MIP-3a, Chemockine (C-C motif) ligand 20, liver activation regulated chemokine, LARC, Macrophage Inflammatory Protein-3ž€, MIP-3-alpha
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
Cat # Size Price Quantity Check Availability Save
534604 100 µg £154.00
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Description

CCL20 (MIP-3α) belongs to the CC chemokine family. Human CCL20 is a protein of 96 amino acids, which shares the highest homology of 28% amino acid identity with MIP-1β and 64% amino acid sequence identity with the mouse CCL20. CCL20 is produced by activated monocytes, T cells, neutrophils, endothelial cells, epithelial cells and fibroblasts. Its expression is induced by lipopolysaccharide (LPS) and inflammatory cytokines such as tumor necrosis factor, IL-1β and interferon-γ, and downregulated by IL-10.

 

CCL20 is the ligand for chemokine receptor CCR6, which is expressed on memory T cells, B cells, and immature dendritic cells (DCs). Therefore, it is involved in the regulation of DC trafficking and recruitment and activation of T cells. CCL20 also has potent antimicrobial activity.

 

Upregulation of CCL20 plays important roles in autoimmune pathogenesis of the central nerve system (MS), pathogenesis of encephalomyelitis, psoriasis, rheumatoid arthritis, and inflammatory bowel diseases. CCL20 is also involved in various cancers, such as gastrointestinal cancers, pancreatic cancer, hepatocellular carcinoma, and breast cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
In-house recombinant human CCL20
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration of 1.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of human CCL20 (MIP-3α) recombinant protein ranging from 7.81 pg/mL to 500 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance.

Application Notes

ELISA Detection: The biotinylated L2418G3 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified L2412F11 antibody (Cat. No. 534502) as the capture antibody.

Note: For testing human CCL20 (MIP-3a) in cell culture supernatant, serum, plasma (EDTA), and other biological fluids, BioLegend's LEGEND MAX™ Kits (Cat. No. 441407 & 441408) are specially developed and recommended.

RRID
AB_2715820 (BioLegend Cat. No. 534604)

Antigen Details

Structure
Protein of 96 amino acid
Distribution

Expression is induced by lipopolysaccharide (LPS) and inflammatory cytokines such as tumor necrosis factor, IL-1β and interferon-γ, and downregulated by IL-10.

Function
Involved in the regulation of DC trafficking and recruitment and activation of T cells. CCL20 also has potent antimicrobial activity
Interaction
CCR6
Ligand/Receptor
Ligand for chemokine receptor CCR6
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Gene ID
6364 View all products for this Gene ID
UniProt
View information about CCL20 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 0    Revision Date: 10/09/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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