Biotin anti-Apo E, 154-162 Antibody

Pricing & Availability
Clone
A17065A (See other available formats)
Other Names
Apolipoprotein E, APO-E, Alzheimer Disease 2, LDLCQ5, APOE, LPG, AD2,
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A17065A_Biotin_ApoE154-162_Antibody_1_100818
Western blot of Biotin anti-Apo E, 154-162 antibody (clone A17065A). Lane 1: Molecular weight marker; Lane 2: 40 ng of Apo E2 recombinant human protein; Lane 3: 40 ng of Apo E3 recombinant human protein; Lane 4: 40 ng of Apo E4 recombinant human protein; Lane 5: 40 µg of human plasma. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Streptavidin (Cat. No. 405210) for 60 minutes at room temperature. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • A17065A_Biotin_ApoE154-162_Antibody_1_100818
    Western blot of Biotin anti-Apo E, 154-162 antibody (clone A17065A). Lane 1: Molecular weight marker; Lane 2: 40 ng of Apo E2 recombinant human protein; Lane 3: 40 ng of Apo E3 recombinant human protein; Lane 4: 40 ng of Apo E4 recombinant human protein; Lane 5: 40 µg of human plasma. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Streptavidin (Cat. No. 405210) for 60 minutes at room temperature. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
Cat # Size Price Quantity Avail. Save
852805 25 µg £77
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852806 100 µg £171
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Description

Apolipoprotein E (Apo E) belongs to a class of apolipoproteins that mediate the binding, internalization, and catabolism of lipoprotein particles. Apo E is produced by the liver and macrophages in peripheral tissues where it regulates cholesterol metabolism. In the central nervous system, Apo E is primarily produced by astrocytes and plays an important role in transporting cholesterol to neurons via Apo E receptors. Apo E has three alleles (Apo E2, Apo E3 and Apo E4) that differ from each other by only one or two amino acids at positions 112 and 158. Apo E4 is one of the largest known genetic risk factors for late-onset Alzheimer's disease. Furthermore, Apo E is associated with cardiovascular disease, Lipoprotein Glomerulopathy and Sea-Blue Histiocyte Disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH conjugated Apo E peptide encompassing amino acids 154-162.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody cross-reacts with Apo E2, Apo E3, and Apo E4.

RRID
AB_2783425 (BioLegend Cat. No. 852805)
AB_2783426 (BioLegend Cat. No. 852806)

Antigen Details

Structure
Human Apo E is a 299 amino acid protein with a molecular mass of 32 kD, and exists as 3 major alleles, Apo E2, Apo E3, and Apo E4.
Distribution

Tissue sources: Ubiquitously expressed. Highest levels in liver, brain, spleen, adrenal gland, and kidney.
Cellular distribution: Plasma membrane, nucleus, endoplasmic reticulum, golgi apparatus, endosome, and extracellular.

Function
Apo E is involved in lipoprotein metabolism as well as the transport of fat-soluble vitamins, and cholesterol. Apo E also forms complexes with amyloid beta peptides leading to its uptake and degradation by microglia.
Interaction
Tau, amyloid beta
Ligand Receptor
LDL receptor, SORL1.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Apolipoproteins
Antigen References
  1. Mahley RW. 2016. Arterioscler Thromb Vasc Biol. 36: 1305-1315.
  2. Riedel BC, et al. 2016 J Steroid Biochem Mol Biol. 160:134-47.
  3. Tai LM, et al. 2016 Acta Neuropathol. 131(5):709-23.
  4. Kim J, et al. 2014. Mol. Cells. 37:767
  5. Holtzman DM, et al. 2012. Cold Spring Harb. Perspect. Med. 2:a006312
Gene ID
348 View all products for this Gene ID
UniProt
View information about Apo E, 154-162 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 10/09/2018

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