Biotin anti-Ankyrin-B Antibody

Pricing & Availability
Clone
N105/17 (See other available formats)
Other Names
Ankyrin-2, ankyrin B, ankyrin, brain, non-erythroid ankyrin, ankyrin-2 nonerythrocytic
Isotype
Mouse IgG2a
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Product Citations
publications
N105-17_Biotin_Ankyrin-B_Antibody_1_052119
IHC staining of Biotin anti-Ankyrin-B antibody (Clone N105/17) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 Streptavidin (Cat. No. 405240) for one hour at room temperature. The slide was mounted with Fluoromount G with DAPI. The image was captured with a 10X objective. Scale Bar: 100 µm
  • N105-17_Biotin_Ankyrin-B_Antibody_1_052119
    IHC staining of Biotin anti-Ankyrin-B antibody (Clone N105/17) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 Streptavidin (Cat. No. 405240) for one hour at room temperature. The slide was mounted with Fluoromount G with DAPI. The image was captured with a 10X objective. Scale Bar: 100 µm
  • N105-17_Biotin_Ankyrin-B_Antibody_2_052119
    IHC staining of Biotin anti-Ankyrin-B antibody (Clone N105/17) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 Streptavidin (Cat. No. 405240) for one hour at room temperature. The slide was mounted with Fluoromount G with DAPI. The image was captured with a 10X objective. Scale Bar: 100 µm
Cat # Size Price Quantity Avail. Save
821505 25 µg £60
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821506 100 µg £108
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Description

Ankyrins are adaptor proteins that mediate the attachment of membrane proteins to the cytoskeleton. Ankyrin-B (Ankyrin-2) is highly expressed in cardiac tissue where it has an essential role in stabilizing ion transporters and channels in cardiomyocytes in the membrane. Mutations in ankyrin-B are associated with cardiac arrhythmia and ankyrin-B may be involved in human heart failure.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 203-496 (ankyrin repeats 6 to 14) of human Ankyrin-B.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Presynaptic proteins
Gene ID
287 View all products for this Gene ID
UniProt
View information about Ankyrin-B on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 05/21/2019

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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