APC/Fire™ 750 anti-mouse GM-CSF Antibody

Pricing & Availability
Clone
MP1-22E9 (See other available formats)
Other Names
Granulocyte/macrophage-colony stimulating factor, CSF-α, Pluripoietin-α, Eosinophil colony stimulating factor (Eo-CSF), Burst promoting activity (BPA)
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
MP1-22E9_APCFire750_GM-CSF_Antibody_071019
Cell Activation Cocktail-stimulated Th2-polarized C57BL/6 splenocytes were intracellularly stained with CD3 FITC and GM-CSF (clone MP1-22E9) APC/Fire™ 750 (left) or rat IgG2a, κ isotype control (right) APC/Fire™ 750.
  • MP1-22E9_APCFire750_GM-CSF_Antibody_071019
    Cell Activation Cocktail-stimulated Th2-polarized C57BL/6 splenocytes were intracellularly stained with CD3 FITC and GM-CSF (clone MP1-22E9) APC/Fire™ 750 (left) or rat IgG2a, κ isotype control (right) APC/Fire™ 750.
See APC/Fire™ 750 spectral data
Cat # Size Price Quantity Avail. Save
505423 25 µg £97
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505424 100 µg £218
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Description

GM-CSF is a hematopoietic factor that is produced by T cells, macrophages, fibroblasts and endothelial cells. This multifunctional cytokine stimulates progenitor cells of neutrophils, eosinophils, and macrophages. GM-CSF is also a differentiation and activating factor for granulocytic and monocytic cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Yeast-expressed, recombinant mouse GM-CSF
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions. The solution is free of unconjugated APC/Fire™ 750 and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

Excitation Laser
Red Laser (633 nm)
Application Notes

ELISA or ELISPOT Capture1,3-5: The purified MP1-22E9 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP1-31G6 antibody (Cat. No. 505502) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.
Flow Cytometry8: The fluorochrome-labeled MP1-22E9 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify GM-CSF -producing cells within mixed cell populations.
Neutralization2-4: The MP1-22E9 antibody can neutralize the bioactivity of natural or recombinant GM-CSF. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for in vivo and in vitro neutralization (Cat. No. 505408).
Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections1,6,7, and immunocytochemistry8.

Application References

(PubMed link indicates BioLegend citation)
  1. Sander B, et al. 1993. J. Immunol. Methods 166:201.
  2. Suda T, et al. 1990. Cell. Immunol. 129:228.
  3. Nozaki S, et al. 1991. J. Invest. Dermatol. 97:10.
  4. Abrams JS, et al. 1992. Immunol. Rev. 127:5.
  5. Abrams JS. 2001. Curr. Protoc. Immunol. Unit 6.20.
  6. Sander B, et al. 1991. Immunol. Rev. 119:65.
  7. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
  8. Larkin J, et al. 2006. J. Immunol. 177:268.

Antigen Details

Structure
Cytokine; 22 kD (Mammalian)
Bioactivity
Growth/development granulocyte/macrophage progenitors; differentiates myeloblasts/monoblasts; synergizes with Epo proliferation of erythroid/megakaryocytic progenitors
Cell Sources
T cells, monocytes/macrophages, fibroblasts, endothelial cells, mast cells
Cell Targets
Granulocyte/macrophage/erythroid/megakaryocytic progenitors, myeloblasts, monoblasts
Receptors
Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131) in common with IL-3R, IL-5R
Biology Area
Cell Biology, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Demetri, G., et al. 1991. Blood 78:2791.
3. Fan, D., et al. 1991. In vivo 5:571.
4. Negrin, R., et al. 1992. Adv. Pharmacol. 23:263.

Regulation
Synergistic with IL-1, IL-3, G-CSF; E21R competitive antagonist for receptor binding; stored in ECM with heparan sulfate proteoglycans
Gene ID
12981 View all products for this Gene ID
UniProt
View information about GM-CSF on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/10/2019

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