APC/Fire™ 750 anti-mouse CD366 (Tim-3) Antibody

Pricing & Availability
Clone
RMT3-23 (See other available formats)
Other Names
T cell immunoglobulin and mucin domain containing 3 protein, hepatitis virus cellular receptor 2, CD366
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
RMT3-23_APCFire750_CD366_Antibody_022416
Mouse Tim-3 transfected cells were stained with anti-mouse CD366 (Tim-3, clone RMT3-23) APC/Fire™ 750 (filled histogram) or rat IgG2a, κ APC/Fire™ 750 isotype control (open histogram).
  • RMT3-23_APCFire750_CD366_Antibody_022416
    Mouse Tim-3 transfected cells were stained with anti-mouse CD366 (Tim-3, clone RMT3-23) APC/Fire™ 750 (filled histogram) or rat IgG2a, κ APC/Fire™ 750 isotype control (open histogram).
See APC/Fire™ 750 spectral data
Cat # Size Price Quantity Avail. Save
119737 25 µg £101
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119738 100 µg £225
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Description

CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on Th1 lymphocytes and CD11b+ macrophages. Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions. The solution is free of unconjugated APC/Fire™ 750 and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for relevant formats) include: in vitro1 and in vivo2 blocking of Tim-3, and immunohistochemical staining of frozen sections2. The LEAF™ purified antibody (Endotoxin EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 119707).

Application References

(PubMed link indicates BioLegend citation)
  1. Nakae S, et al. 2007. Blood 110(7):2565-8. (FC, Block)
  2. Oikawa T, et al. 2006. J. Immunol. 177(7):4281-7. (FC, Block, IHC)

Antigen Details

Structure
Transmembrane protein containing immunoglobulin domain and mucin-like domain; predicted molecular weight 31 kD; can exist as a soluble form lacking mucin and transmembrane domains
Distribution

Expressed on activated Th1 lymphocytes and CD11b+ macrophages

Function
May play a role in the development of immune responses and the development of Th1-mediated responses
Ligand/Receptor
Putative ligand on resting CD4+ lymphocytes
Cell Type
Macrophages, Th1
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Sabatos CA, et al. 2003. Nat. Immunol. 4:1102.
2. Sanchez-Fueyo A, et al. 2003. Nat. Immunol. 4:1102.
3. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454.
4. Mooney L, et al. 2002. Nature 415:536.

Gene ID
171285 View all products for this Gene ID
UniProt
View information about CD366 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/13/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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