APC/Fire™ 750 anti-mouse CD1d (CD1.1, Ly-38) Antibody

Pricing & Availability
Clone
1B1 (See other available formats)
Other Names
CD1, CD1.1, Ly-38
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
1B1_APCFire750_CD1d_Antibody_092818
C57BL/6 mouse splenocytes were stained with B220 FITC and CD1d APC/Fire™ 750 (clone 1B1, left) or Rat IgG2b, κ isotype control APC/Fire™ 750 (right).
  • 1B1_APCFire750_CD1d_Antibody_092818
    C57BL/6 mouse splenocytes were stained with B220 FITC and CD1d APC/Fire™ 750 (clone 1B1, left) or Rat IgG2b, κ isotype control APC/Fire™ 750 (right).
See APC/Fire™ 750 spectral data
Cat # Size Price Quantity Avail. Save
123525 25 µg £71
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123526 100 µg £195
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Description

CD1d, known as CD1.1 and Ly-38, is a 48 kD type I membrane glycoprotein with structural similarities to MHC class I and is non-covalently associated with β2-microglobulin. In humans, CD1 family consists of group I proteins (CD1a, CD1b, and CD1c), group II (CD1d), and group III (CD1e). But CD1d is the only CD1 molecule has been found in mouse. Mouse CD1d has broad tissue distribution, and is found on leukocytes, dendritic cells, epithelial cells, and thymocytes. CD1d plays a role in non-peptide glycolipid antigen presentation to CD1d-restricted T cells. It has been shown that PKCδ is a critical regulator of CD1d-mediated antigen presentation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Cd1.1 cDNA-transfected RMA-S mouse T lymphoma
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions. The solution is free of unconjugated APC/Fire™ 750 and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemistical staining, and blocking function3.
 

This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact the Regents of the University of California.

Application References

(PubMed link indicates BioLegend citation)
  1. Fischer K, et al. 2004. P. Natl. Acad. Sci. USA 101:10685. (Block)
  2. Brozovic S, et al. 2004. Nat. Med. 10:535.
  3. Brossay L, et al. 1997. J. Immunol.. 159:1216. (Block)
  4. Jiang J, et al. 2012. PLoS One. 7:47487. PubMed
RRID
AB_2783067 (BioLegend Cat. No. 123525)
AB_2783068 (BioLegend Cat. No. 123526)

Antigen Details

Structure
48 kD glycoprotein, Ig superfamily
Distribution

leukocytes, dendritic cells, epithelial cells, thymocytes

Function
Antigen presentation
Ligand Receptor
similar to MHC class I, associate with ß-microglobulin
Cell Type
Dendritic cells, Epithelial cells, Leukocytes, Thymocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Brudin N, et al. 1998. J. Immunol. 161:3271.
2. Amano M, et al. 1998. J. Immunol. 161:1710.
3. Brossay L, et al. 1997. J. Immunol. 159:1216.
4. Dougan SK, et al. 2007. Curr. Top. Microbiol. Immunol. 314:113.
5. Brutkiewicz RR, et al. 2007. Eur. J. Immunol. 37:2390.

Gene ID
12479 View all products for this Gene ID
UniProt
View information about CD1d on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/02/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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