APC/Fire™ 750 anti-human β2-microglobulin Antibody

Pricing & Availability
Clone
A17082E (See other available formats)
Other Names
beta2-microglobulin b2-M, b2M
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A17082E_APCFire750_beta2-microglobulin_Antibody_060619.png
Human peripheral blood lymphocytes were stained with β2-microglobulin (clone A17082E) APC/Fire™ 750 (filled histogram) or mouse IgG1, κ APC/Fire™ 750 isotype control (open histogram).
  • A17082E_APCFire750_beta2-microglobulin_Antibody_060619.png
    Human peripheral blood lymphocytes were stained with β2-microglobulin (clone A17082E) APC/Fire™ 750 (filled histogram) or mouse IgG1, κ APC/Fire™ 750 isotype control (open histogram).
See APC/Fire™ 750 spectral data
Cat # Size Price Quantity Avail. Save
395807 25 tests £101
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395808 100 tests £218
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Description

β2-microglobulin (β2M) is a 12 kD nonpolymorphic Ig like protein. It is a non-membrane-anchored glycoprotein and is noncovalently associated with 39-44 kD polymorphic heavy chains of MHC class I molecules to form HLA class I antigen complex. In association with HLA class I, β2M is expressed on all leukocytes, platelets, endothelial cells, and epithelial cells. β2M plays an essential role both in governing MHC class I molecules stability and in promoting antigen binding and presenting the antigen to CD3/TCR complex of CD8+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Beta2-microglobulin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions. The solution is free of unconjugated APC/Fire™ 750 and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

Excitation Laser
Red Laser (633 nm)
Application Notes

Based on in-house testing, staining for clone A17082E is not blocked by clone W6/32 (anti-HLA-A,B,C) and is only partially blocked by clone 2M2 also raised against human β2-microglobulin.

Antigen Details

Structure
12KD, Ig like structure
Distribution

Leukocytes, platelets, endothelial cells, epithelial cells and other nucleated cells.

Function
Form MHC class I
Interaction
MHC class I heavy chain and other MHC I like molecules.
Cell Type
Endothelial cells, Epithelial cells, Leukocytes, Platelets
Antigen References
  1. Engelhard VH. 1994. Curr Opin Immunol. 6:13.
  2. Williams DB, et al. 1989. J Immunol. 142:2796.
  3. Danliczyk UG and TL. Delovitch. 1994. J Immunol. 153:3533.
  4. Williams A, et al. 2002. Tissue Antigens. 59:3.
Gene ID
567 View all products for this Gene ID
UniProt
View information about beta2-microglobulin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/06/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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