APC/Cyanine7 anti-human CD257 (BAFF, BLYS) Antibody

Pricing & Availability
Clone
1D6 (See other available formats)
Workshop
HCDM listed
Other Names
BAFF, BLYS, TNFSF13b, B cell activating factor, tumor necrosis factor ligand superfamily 13B, B lymphocyte stimulator (BLYS), TNF and APOL-related leukocyte expressed ligand 1 (TALL1), TNF homolog that activates apoptosis, NF-κB and JNK (THANK)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1D6_CD257_APC_CYANINE7_Antibody_091319
Human CD257-transfected bovine coronary artery endothelial cell line (B300) was stained with anti-human CD257 (clone 1D6) APC/Cyanine7 (filled histogram) or mouse IgG1, κ APC/Cyanine7 isotype control (open histogram).
  • 1D6_CD257_APC_CYANINE7_Antibody_091319
    Human CD257-transfected bovine coronary artery endothelial cell line (B300) was stained with anti-human CD257 (clone 1D6) APC/Cyanine7 (filled histogram) or mouse IgG1, κ APC/Cyanine7 isotype control (open histogram).
Cat # Size Price Quantity Avail. Save
366511 25 tests £104
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366512 100 tests £218
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Description

BAFF, also known as B cell activating factor, tumor necrosis factor ligand superfamily 13B, B lymphocyte stimulator (BLYS), TNF and APOL-related leukocyte expressed ligand 1 (TALL1), and TNF homolog, activates apoptosis, NF-κB, and JNK (THANK). BAFF is a type II transmembrane protein and is a member of the tumor necrosis factor ligand superfamily. BAFF can be proteolytically cleaved to form a soluble protein. BAFF, like other TNF ligand family members, forms homotrimers. The predicted molecular weight of a BAFF monomer is approximately 31 kD. BAFF is expressed on peripheral blood B and T lymphocytes, monocytes, macrophages, and dendritic cells. It is upregulated by IFN-γ and downregulated by PMA/ionomycin treatment.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CD257 transfected murine cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions. The solution is free of unconjugated APC/Cyanine7 and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Hase H, et al. 2004. Blood 6:2257. (IHC)
RRID
AB_2814320 (BioLegend Cat. No. 366511)
AB_2814321 (BioLegend Cat. No. 366512)

Antigen Details

Structure
Type II transmembrane protein and is a member of the tumor necrosis factor ligand superfamily. It can be proteolytically cleaved to form a soluble protein and it forms homotrimers. The predicted molecular weight of monomer is approximately 31 kD.
Distribution

Peripheral blood B and T lymphocytes, monocytes, macrophages, and dendritic cells. Upregulated by IFN-γ and is downregulated by PMA/ionomycin treatment.

Function
Stimulates B and T cell immunity, regulates humoral immunity by binding TACI and BCMA receptors, and prevents apoptosis and promotes B cell survival by binding the BAFF-specific receptor (BAFFR/BR3).
Ligand/Receptor
BAFF binds to TACI (TNFRSF13B) and BCMA (TNFRSF17) as well as the APRIL ligand. BAFF also binds to the BAFF-specific receptor (BAFFR/BR3).
Cell Type
B cells, Dendritic cells, Macrophages, Monocytes, T cells
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Signal Transduction, Stem Cells
Molecular Family
CD Molecules, Cytokines/Chemokines, Growth Factors, Soluble Receptors
Antigen References

1. Schneider P, et al. 1999. J. Exp. Med. 189:1747.
2. Moore PA, et al. 1999. Science 285:260.
3. Shu HB, et al. 1999. J. Leuko. Biol. 65:680.
4. Yu G, et al. 2000. Nat. Immunol. 1:252.
5. Oren DA, et al. 2002. Nat. Struct. Biol. 9:288.

Gene ID
10673 View all products for this Gene ID
UniProt
View information about CD257 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/13/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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