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APC/Fire™ 750 anti-human CD36 Antibody

Pricing & Availability
Clone
5-271 (See other available formats)
Regulatory Status
RUO
Other Names
GPIIIb, GPIV
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
5-271_APCFire750_CD36_Antibody_FC_092216
Human peripheral blood platelets were stained with CD36 (clone 5-271) APC/Fire™ 750 (filled histogram) or mouse IgG2a, κ APC/Fire™ 750 isotype control (open histogram).
  • 5-271_APCFire750_CD36_Antibody_FC_092216
    Human peripheral blood platelets were stained with CD36 (clone 5-271) APC/Fire™ 750 (filled histogram) or mouse IgG2a, κ APC/Fire™ 750 isotype control (open histogram).
Compare all formats See APC/Fire™ 750 spectral data
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336219 25 tests £109
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336220 100 tests £241
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Description

CD36 is an 85 kD integral membrane glycoprotein, also known as GPIIIb, or GPIV. It is expressed on various epithelial and endothelial cells as well as erythrocytes, platelets, macrophages/monocytes and some macrophage-derived dendritic cells. CD36 functions as a scavenger receptor, binding thrombospondin, long chain fatty acids, oxidized LDL, collagen type I, IV, and V as well as apoptotic cells. The 5-271 antibody has been reported to be useful for flow cytometry.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human platelets
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence2.

Application References

(PubMed link indicates BioLegend citation)
  1. Stelner E, et al. 2006. J. Cell Sci. 119:459.
  2. Stewart DA, et al. 2012. Mol. Cancer Res. 10:727. (IF)
Product Citations
  1. Hofbauer D, et al. 2021. Immunity. 54(8):1772-1787.e9. PubMed
  2. Herati RS, et al. 2022. Nat Immunol. 23:1183. PubMed
RRID
AB_2629775 (BioLegend Cat. No. 336219)
AB_2629776 (BioLegend Cat. No. 336220)

Antigen Details

Structure
Integral membrane protein, CD36 family, single chain glycoprotein, 85-113 kD
Distribution

Expressed in various epithelial and endothelial cells, erythrocytes, platelets, monocytes/ macrophages, some macrophage-derived dendritic cells

Function
Scavenger receptor for thrombospondin, long chain fatty acids, anionic phospholipids, oxidized LDL, collagen types I, IV and V. Involved in recognition and phagocytosis of apoptotic cells, cell adhesion.
Ligand/Receptor
Thrombospondin, anionic phospholipids, oxidized LDL, collage types I, IV, and V
Cell Type
Dendritic cells, Endothelial cells, Epithelial cells, Erythrocytes, Macrophages, Monocytes, Platelets
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Hogg N, et al. 1984. Immunology 53-753.
2. Greenwalt DE, et al. 1992. Blood 80:1105.
3. Armsesilla AL, et al.1994. J. Biol. Chem. 269:18985.
4. Endemann G, et al. 1993. J. Biol. Chem. 268:11811.

Gene ID
948 View all products for this Gene ID
UniProt
View information about CD36 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/22/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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