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APC/Cyanine7 anti-human IL-4 Antibody

Pricing & Availability
Clone
MP4-25D2 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-4, Ia inducing factor (IaIF), B-cell stimulating factor-1 (BSF-1), Hodgkin's cell growth factor (HCGF), Mast cell growth factor-2 (MCGF-2), Macrophage fusion factor (MFF), T cell growth factor-2 (TCGF-2)
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
MP4-25D2_APCCyanine7_IL4_Antibody_1_060519
PMA + Ionomycin-stimulated (6 hours) human peripheral blood lymphocytes (in the presence of monesin) were surface stained with CD3 PE, fixed, permeabilized, and then stained with IL-4 (clone MP4-25D2) APC/Cyanine7 (left) or rat IgG1, ? APC/Cyanine7 isotype control (right).
  • MP4-25D2_APCCyanine7_IL4_Antibody_1_060519
    PMA + Ionomycin-stimulated (6 hours) human peripheral blood lymphocytes (in the presence of monesin) were surface stained with CD3 PE, fixed, permeabilized, and then stained with IL-4 (clone MP4-25D2) APC/Cyanine7 (left) or rat IgG1, ? APC/Cyanine7 isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
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500833 25 tests £121
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500834 100 tests £262
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Description

IL-4 is a pleiotropic cytokine that is produced by activated T cells, mast cells, and basophils. IL-4 elicits many different biological responses but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL-4, IL-5, IL-10, and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. The second is regulating IgE and IgG1 production by B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Pig, Rhesus
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CHO-expressed, recombinant human IL-4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

ELISA Detection1,3 or ELISPOT Detection4,5: The biotinylated MP4-25D2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 8D4-8 antibody (Cat. No. 500702/500707) as the capture antibody.
Flow Cytometry6,9: The fluorochrome-labeled MP4-25D2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-4 -producing cells within mixed cell populations.
Neutralization1-3: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-4 bioactivity (Cat. No. 500815). The MP4-25D2 antibody can neutralize the bioactivity of natural or recombinant IL-4.

Additional Product Notes

BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Chretien I, et al. 1989. J. Immunol. Methods 117:67. (ELISA Detection, Neut)
  2. Ramanathan L, et al. 1993. Biochem. 32:3549. (Neut)
  3. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA Detection, Neut)
  4. Mahanty S, et al. 1992. J. Immunol. 148:3567. (ELISPOT Detection)
  5. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. (ELISPOT Detection)
  6. Prussin C, et al. 1995. J. Immunol. Methods 188:117. (ICFC)
  7. Raqib R, et al. 1995. Infect. Immun. 63:289.
  8. Andersson J, et al. 1994. Immunology 83:16.
  9. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (ICFC) PubMed
  10. Kubota M, et al. 1997. J. Immunol. 158:5321.
  11. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. PubMed
  12. Kroneke MA, et al. 2012. J. Immunol. 188:3734. PubMed
Product Citations
  1. Salehi Z, et al. 2016. PLoS One. 11: 0159565. PubMed
  2. Trotta E, et al. 2018. Nat Med. 24:1005. PubMed
  3. Dai G, et al. 2021. JCI Insight. 6:. PubMed
  4. Zhang JA, et al. 2020. Front Immunol. 11:518. PubMed
RRID
AB_2616757 (BioLegend Cat. No. 500833)
AB_2616758 (BioLegend Cat. No. 500834)

Antigen Details

Structure
Cytokine; 15-19 kD (Mammalian)
Bioactivity
Differentiation of naïve CD4+ T cells to the TH2 type, proliferation/differentiation of activated B cells, expression of class II MHC antigens, and of low affinity IgE receptors in resting B cells
Cell Sources
Mast cells, T cells, bone marrow stromal cells
Cell Targets
B cells, T cells, monocytes, endothelial cells, fibroblasts
Receptors
Heterodimer IL-4Rα (CD124); γ-subunit (CD132) in common with IL-2R, IL-7R, IL-13R, IL-15R
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Boulay J, et al. 1992. Curr. Opin. Immunol. 4:294.
3. Dullens H, et al. 1991. In vivo 5:567.
4. Paul W. 1991. Blood 77:1859.

Regulation
Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
Gene ID
3565 View all products for this Gene ID
UniProt
View information about IL-4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/05/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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