APC/Cyanine7 anti-human CD273 (B7-DC, PD-L2) Antibody

Pricing & Availability
Clone
MIH18 (See other available formats)
Workshop
HCDM listed
Other Names
B7-DC, PD-L2, PDL2, B7DC
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
MIH18_APCCyanine7_CD273_Antibody_010819_updated.png
Human monocyte-derived dendritic cells were stained with CD273 (B7-DC, PD-L2)(clone MIH18) APC/Cyanine7 (filled histogram) or Mouse IgG1, κ APC/Cyanine7 isotype control (open histogram)
  • MIH18_APCCyanine7_CD273_Antibody_010819_updated.png
    Human monocyte-derived dendritic cells were stained with CD273 (B7-DC, PD-L2)(clone MIH18) APC/Cyanine7 (filled histogram) or Mouse IgG1, κ APC/Cyanine7 isotype control (open histogram)
Cat # Size Price Quantity Avail. Save
345515 25 tests £87
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345516 100 tests £215
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Description

CD273, known as B7-DC, is also called programmed death ligand 2 (PDL2). This ligand is a 25 kD type I transmembrane protein and a member of B7 family within the immunoglobulin receptor superfamily and is expressed on a subset of dendritic cells, liver and a small subset of macrophages as well as a few transformed cell lines. CD273 has been reported to be stimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engaging the PD-1 receptor. CD273 has also been reported to bind to an alternative receptor and to mediate T cell activation through such non-PD1 mediated interactions. Clone MIH18 is reported to block PDL2.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human B7-DC transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions. The solution is free of unconjugated APC/Cyanine7 and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking4,5, and immunohistochemistry in frozen sections2 and paraffin-embedded formalin-fixed sections6. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 345504).

Additional Product Notes

BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Youngnak-Piboonratankit P, et al. 2004. Immunol. Lett. 94:215. (IHC, IF)
  2. Ohigashi Y, et al. 2005. Clin. Cancer. Res. 8:2947. (IHC, IF)
  3. Hobo W, et al. 2010. Blood 25:4501. (FC)
  4. Nagamatsu T, et al. 2009. Hum. Reprod. 24:3160. (Block)
  5. Alvarez IB, et al. 2010. J. Infect. Dis. 202:524. (Block)
  6. Taube JM, et al. 2014. Clin. Cancer Res. 19:5064. (IHC) PubMed
RRID
AB_2783233 (BioLegend Cat. No. 345515)
AB_2783234 (BioLegend Cat. No. 345516)

Antigen Details

Structure
B7 Immunoglobulin superfamily, 25 kD
Distribution

Dendritic cells, liver, few transformed cell lines, subset of macrophages

Function
Binds to PD-1 and alternative receptor; ligation on DC stimulates, inhibits T cell responses via PD-1 binding, stimulates T cells via alternative receptor binding and promotes tumor immunity
Ligand Receptor
PD-1
Cell Type
Dendritic cells, Macrophages
Biology Area
Immunology
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.
2. Ohigashi Y, et al. 2005. Clin. Cancer. Res. 8:2947.

Gene ID
80380 View all products for this Gene ID
UniProt
View information about CD273 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 01/08/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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