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APC/Cyanine7 anti-human CD14 Antibody

Pricing & Availability
Clone
HCD14 (See other available formats)
Regulatory Status
RUO
Other Names
LPS receptor
Isotype
Mouse IgG1, κ
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Product Citations
publications
HCD14_APCCyanine7_CD46_Antibody_062119
Human peripheral blood monocytes stained with CD14 (clone HCD14) APC/Cyanine7 (filled histogram), or Mouse IgG1, ? APC/Cyanine7 isotype (open histogram).
  • HCD14_APCCyanine7_CD46_Antibody_062119
    Human peripheral blood monocytes stained with CD14 (clone HCD14) APC/Cyanine7 (filled histogram), or Mouse IgG1, ? APC/Cyanine7 isotype (open histogram).
Compare all formats See APC/Cyanine7 spectral data
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325619 25 tests £105
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325620 100 tests £213
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Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Product Citations
  1. Beitnes AC, et al. 2012. PLoS One. 7:e33556. PubMed
  2. Sun R, et al. 2021. Cell Reports. 36(7):109559. PubMed
  3. Lin L, et al. 2020. NAR Genom Bioinform. 2:lqaa059. PubMed
  4. Carre C, et al. 2021. iScience. 24:102970. PubMed
  5. Zong D, et al. 2021. BMC Biol. 19:79. PubMed
  6. Manukyan G, et al. 2020. Auto Immun Highlights. 11:5. PubMed
  7. Rajasekhar M, et al. 2017. J Autoimmun. 10.1016/j.jaut.2017.01.002. PubMed
  8. AC Belkina, JE Snyder-Cappione 2017. Cytometry A. 91:175-179. PubMed
  9. Soriano-Sarabia N, et al. 2012. J Immunol. 189:5212. PubMed
  10. Palamides P, et al. 2016. Dis Model Mech. 9: 985 - 997. PubMed
  11. Hagen SH, et al. 2020. Cell Rep. 33:108485. PubMed
  12. Yang Z, et al. 2018. Front Immunol. 9:2613. PubMed
  13. Weisberg SP, et al. 2020. Cell Reports. 29(12):3916-3932.e5.. PubMed
  14. Liu C, et al. 2021. Cell Res. 31:1106. PubMed
  15. Tiwari-Heckler S, et al. 2021. Cell Rep. 37:109897. PubMed
  16. Zhou Y, et al. 2017. Front Cell Infect Microbiol. 7:457. PubMed
  17. Nicholas DA et al. 2019. Cell Metab. 30(3):447-461 . PubMed
  18. Ma YV, et al. 2021. MAbs. 13:2003281. PubMed
  19. Cui L, et al. 2021. Am J Reprod Immunol. 86:e13436. PubMed
  20. Moquin-Beaudry G, et al. 2022. Cell Rep Methods. 2:100153. PubMed
  21. Kuebler P, et al. 2015. Proc Natl Acad Sci U S A. 112: 8379 - 8384. PubMed
  22. Evans RDR, et al. 2020. Nat Commun. 3.491666667. PubMed
  23. Bharath LP, et al. 2020. Cell Metabolism. 32(1):44-55.e6.. PubMed
  24. Schrøder M, et al. 2016. PLoS One. 11: 0157387. PubMed
  25. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  26. Markman JL, et al. 2020. Nat Commun. 11:1613. PubMed
  27. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  28. Kacherovsky N, et al. 2019. Nat Biomed Eng. 0.66875. PubMed
  29. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
  30. Port JR, et al. 2020. J Virol. 94:. PubMed
  31. Hagen SH, et al. 2021. STAR Protocols. 2(3):100641. PubMed
  32. Samarani S, et al. 2020. Mediators Inflamm. 2020:6401969. PubMed
  33. Zhuang Q, et al. 2021. Front Pharmacol. 12:706580. PubMed
  34. Bravo-Flores E, et al. 2018. Int J Mol Sci. 19:E1074. PubMed
  35. Roy Chowdhury R, et al. 2018. Nature. 560:644. PubMed
  36. Ravenhill BJ, et al. 2020. Sci Rep. 10:4560. PubMed
  37. Körner C et al. 2017. Cell host & microbe. 22(1):111-119 . PubMed
  38. Nakajima S, et al. 2021. Mol Cancer Res. 19:1085. PubMed
  39. Kubala MH, et al. 2018. Cell Rep. 25:2177. PubMed
  40. He L, et al. 2022. EMBO Mol Med. 14:e14990. PubMed
  41. Wang M, et al. 2021. iScience. 24(7):102766. PubMed
  42. Chakraborty A, et al. 2022. Methods Mol Biol. 2442:565. PubMed
  43. Friedensohn S, et al. 2018. Front Immunol. 9:1401. PubMed
RRID
AB_830692 (BioLegend Cat. No. 325619)
AB_830693 (BioLegend Cat. No. 325620)

Antigen Details

Structure
GPI-linked membrane glycoprotein, 53-55 kD
Distribution

Monocytes, macrophages, granulocytes (low)

Function
LPS receptor, clearance of Gram-negative pathogens
Ligand/Receptor
LPS
Cell Type
Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.

Gene ID
929 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 06/21/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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