APC anti-rat CD25 Antibody

Pricing & Availability
Clone
OX-39 (See other available formats)
Other Names
IL-2 receptor chain α, IL-2Rα, low affinity IL-2 receptor chain, Tac, p55, Ly-43
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
OX-39_APC_CD25_Antibody_071719
Con A activated (3-days) rat splenocytes were stained with CD25 (clone OX-39) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
  • OX-39_APC_CD25_Antibody_071719
    Con A activated (3-days) rat splenocytes were stained with CD25 (clone OX-39) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
See APC spectral data
Cat # Size Price Quantity Avail. Save
202113 25 µg £54
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202114 100 µg £128
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Description

CD25 is a 55 kD glycoprotein also known as IL-2 receptor α chain. It is broadly expressed on activated T and B cells, a subset of thymic and splenic dendritic cells, and intestinal epithelial cells in the rat. IL-2 is a critical cytokine involved in lymphocyte proliferation and clonal expansion. IL-2 signaling requires the high affinity IL-2 receptor composed of IL-2 receptor chains α, β, and γ. The OX-39 antibody weakly inhibits IL-2 binding to the IL-2 receptor and has been shown to block some IL-2 mediated responses in vitro.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Rat T cell blasts from mixed lymphocyte reactions
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistryof acetone-fixed frozen sections1, immunoprecipitation1, weakly blocks IL-2 binding1, 2, 3, and blocks IL-2 induced epithelial cell migration3.

Application References

(PubMed link indicates BioLegend citation)
  1. Peterson DJ, et al. 1987. Mol. Immunol. 24:1281. (IHC, IP, Block)
  2. Tellides G, et al. 1987. Transplant Proc. 19:4231. (Block)
  3. Digness AU, et al. 1996. Exp.Cell Res. 225:422. (Block)

Antigen Details

Structure
Type I membrane glycoprotein, 55 kD
Distribution

Activated T cells and B cells, thymic and splenic dendritic cells, intestinal epithelial cells

Function
Low affinity receptor for IL-2; associates with IL-2 receptor β (CD122) and γ (CD132) chains to form high affinity receptor
Ligand/Receptor
IL-2
Cell Type
B cells, Dendritic cells, Epithelial cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Digness AU, et al. 1996. Exp.Cell Res. 225:422.
2. Peterson DJ, et al. 1987. Mol. Immunol. 24:1281.

Gene ID
16184 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/17/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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