- OX-39 (See other available formats)
- Other Names
- IL-2 receptor chain α, IL-2Rα, low affinity IL-2 receptor chain, Tac, p55, Ly-43
- Mouse IgG1, κ
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- Product Citations
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CD25 is a 55 kD glycoprotein also known as IL-2 receptor α chain. It is broadly expressed on activated T and B cells, a subset of thymic and splenic dendritic cells, and intestinal epithelial cells in the rat. IL-2 is a critical cytokine involved in lymphocyte proliferation and clonal expansion. IL-2 signaling requires the high affinity IL-2 receptor composed of IL-2 receptor chains α, β, and γ. The OX-39 antibody weakly inhibits IL-2 binding to the IL-2 receptor and has been shown to block some IL-2 mediated responses in vitro.Product Details
- Antibody Type
- Host Species
- Rat T cell blasts from mixed lymphocyte reactions
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemistryof acetone-fixed frozen sections1, immunoprecipitation1, weakly blocks IL-2 binding1, 2, 3, and blocks IL-2 induced epithelial cell migration3.
(PubMed link indicates BioLegend citation)
- Peterson DJ, et al. 1987. Mol. Immunol. 24:1281. (IHC, IP, Block)
- Tellides G, et al. 1987. Transplant Proc. 19:4231. (Block)
- Digness AU, et al. 1996. Exp.Cell Res. 225:422. (Block)
- Type I membrane glycoprotein, 55 kD
Activated T cells and B cells, thymic and splenic dendritic cells, intestinal epithelial cells
- Low affinity receptor for IL-2; associates with IL-2 receptor β (CD122) and γ (CD132) chains to form high affinity receptor
- Cell Type
- B cells, Dendritic cells, Epithelial cells, T cells
- Biology Area
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
1. Digness AU, et al. 1996. Exp.Cell Res. 225:422.
2. Peterson DJ, et al. 1987. Mol. Immunol. 24:1281.
- Gene ID
- 16184 View all products for this Gene ID
- View information about CD25 on UniProt.org
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