- RMP-1 (See other available formats)
- Regulatory Status
- Other Names
- GMP-140, Platelet activation-dependent granule membrane protein (PADGEM)
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD62P is a 140 kD type I transmembrane glycoprotein, also known as P-selectin, platelet activation-dependent granule membrane protein (PADGEM), and GMP-140. It is expressed on activated platelets, megakaryocytes, and endothelial cells. CD62P is primarily stored in secretory α-granules in platelets and Weibel-Palade bodies in endothelial cells, and is rapidly relocated to the plasma membrane upon activation. The ligands for CD62P are CD162 and CD24. A primary function of CD62P is cell adhesion during neutrophil rolling, and platelet-neutrophil and platelet-monocyte interactions.Product Details
- Mouse, Rat
- Antibody Type
- Host Species
- Thrombin activated rat platelets
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunofluorescence6, immunohistochemistry5, ELISA7, Western blotting4, immunoprecipitation4, and blocking in rats1,2 and mice3.
(PubMed link indicates BioLegend citation)
- Walter UM, et al. 1997. Immunology. 92:290. (Block-rats)
- Johnston B, et al. 1997. J. Immunol. 159:4514. (Block-rats)
- Hickey MJ, et al. 1998. Circ. Res. 83:1124. (Block-mice)
- Walter UM, et al. 1997. Hybridoma. 16:249. (WB, IP)
- Alvarez A, et al. 2004. Blood 104:402. (IHC)
- Piccio L, et al. 2002. J. Immunol. 168:1940. (IF)
- Walter UM, et al. 1997. Hybridoma. 16:249. (ELISA)
- Product Citations
AB_2565272 (BioLegend Cat. No. 148303)
AB_2565273 (BioLegend Cat. No. 148304)
- Type I transmembrane glycoprotein, 140 kD
Activated platelets, megakaryocytes, endothelial cells
- Adhesion, neutrophil rolling, platelet-neutrophil and platelet-monocyte interactions
- CD162 (PSGL-1), CD24 and sialylated Lewis X
- Cell Type
- Endothelial cells, Megakaryocytes, Platelets
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. McEver R, et al. 1995. J. Biol. Chem. 270:11025.
2. Varki A. 1994. Proc. Natl. Acad. Sci. USA 91:7390.
- Gene ID
- 20344 View all products for this Gene ID 25651 View all products for this Gene ID
- View information about CD62P on UniProt.org
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