APC anti-mouse Ly49H Antibody

Pricing & Availability
Clone
3D10 (See other available formats)
Other Names
Klra8, Cmv1
Isotype
Mouse IgG1, κ
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Product Citations
publications
3D10_APC_Ly49H _Antibody_100318
C57BL/6 mouse splenocytes were stained with CD49b (clone DX5) PE and Ly49H (clone 3D10) APC (left) or Mouse IgG1, κ APC Isotype Control (right).
  • 3D10_APC_Ly49H _Antibody_100318
    C57BL/6 mouse splenocytes were stained with CD49b (clone DX5) PE and Ly49H (clone 3D10) APC (left) or Mouse IgG1, κ APC Isotype Control (right).
See APC spectral data
Cat # Size Price Quantity Avail. Save
144711 25 µg £64
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144712 100 µg £144
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Description

Ly49H, also known as Cmv1 and Klra8, is a 28.3 kD protein.  It is expressed on NK cells (but not on NKT cells) from C57BL/6 mice (but not Balb/c or DBA/2 mice).  Expression of Ly49H has been associated with resistance to MCMV infection in C57BL/6 mice.  Modulation of Ly49H expression by its antibody made MCMV-resistant C57BL/6 mice susceptible to infection.  Unlike Ly49A, Ly49H lacks the ITIM (immunoreceptor tyrosine-based inhibitory motif) but has ITAM (immunoreceptor tyrosine-based activation motif), which is associated with DAP-12 (KARAP, killer cell activating motif). 

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C1498-Ly49A/H transfectant
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats of this clone) include: immunoprecipation1, and in vitro2 and in vivo3 blocking activity.  The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 144704).

Application References

(PubMed link indicates BioLegend citation)
  1. Guseva NV, et al. 2010. Eur. J. Immunol. 40:2618. (IP)
  2. Orr MT, et al. 2009. J. Exp. Med. 206:807. (Block)
  3. Rodriguez M, et al. 2004. J. Immunol. 173:6312. (Block)
RRID
AB_2783110 (BioLegend Cat. No. 144711)
AB_2783111 (BioLegend Cat. No. 144712)

Antigen Details

Structure
28.3 kD
Distribution

NK cells but not NKT cells

Function
Interacts with m157, a cytomegalovirus-encoded protein
Ligand Receptor
m157
Biology Area
Immunology, Innate Immunity
Antigen References

1. Gosselin P, et al. 1999. J. Leuko. Bio. 66:165.
2. Lee SH, et al. 2001. Nat. Genet. 28:42.
3. Daniels KA, et al. 2001. J. Exp. Med. 194:29.

Gene ID
16639 View all products for this Gene ID
UniProt
View information about Ly49H on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 10/04/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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