APC anti-mouse IL-2 Antibody

Pricing & Availability
Clone
JES6-5H4 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-2, T cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)
Isotype
Rat IgG2b, κ
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Product Citations
publications
JES6-5H4_APC_122107
PMA+ionomycin stimulated C57BL/6 mouse splenocytes (6 hours) stained with anti-CD3 PE (17A2) and intracellularly stained with JES6-5H4 APC
  • JES6-5H4_APC_122107
    PMA+ionomycin stimulated C57BL/6 mouse splenocytes (6 hours) stained with anti-CD3 PE (17A2) and intracellularly stained with JES6-5H4 APC
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503809 25 µg £68
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503810 100 µg £181
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Description

IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli-expressed, recombinant mouse IL-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

ELISA Detection1-3 or ELISPOT Detection4-6: The biotinylated JES6-5H4 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JES6-1A12 antibody (Cat. Nos. 503701 & 503702) as capture antibody and recombinant mouse IL-2 (Cat. No. 575409) as the standard.
Flow Cytometry8-10: The fluorochrome-labeled JES6-5H4 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-2 -producing cells within mixed cell populations.
Neutralization1,7: The Ultra-LEAF™ purified antibody (Endotoxin in vivo and in vitro (Cat. No. 503845-503850)) is recommended for neutralization.
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections2, in vivo capture7, and immunocytochemistry.
Note: For testing mouse IL-2 in serum, plasma or supernatant, BioLegend's ELISA MAX™ Sets (Cat. No. 431001 & 431004) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5.
  2. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
  3. Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.20.
  4. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19.
  5. Mo X, et al. 1995. J. Virol. 69:1288.
  6. Karulin A, et al. 2000. J. Immunol. 164:1862.
  7. Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons New York. Unit 6.28.
  8. Ko SY, et al. 2005. J. Immunol. 175:3309. PubMed
  9. Kang SS and Allen PM. 2005. J. Immunol. 174:5382.
  10. Lawson BR, et al. 2007. J. Immunol. 178:5366.
Product Citations
  1. Caslin HL, et al. 2023. Front Immunol. 13:984859. PubMed
  2. Wu Y, et al. 2023. Nat Cancer. 4:382. PubMed
  3. Read KA, et al. 2023. Nat Commun. 14:1652. PubMed
  4. Park HJ, et al. 2023. NPJ Vaccines. 8:84. PubMed
  5. Qian Y, et al. 2021. Cell Reports. 36(8):109602. PubMed
  6. Maschmeyer P, et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.11.005. PubMed
  7. Ma Z, et al. 2014. PLoS One. 9:112292. PubMed
  8. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  9. Marangoni F, et al. 2021. Cell. . PubMed
  10. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  11. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  12. Tritama E, et al. 2018. Hum Vaccin Immunother. 14:1524. PubMed
  13. Hanna CC, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  14. Burrack K, et al. 2015. J Immunol. 194:678. PubMed
  15. Chauveau L, et al. 2021. EMBO Rep. 22:e52447. PubMed
  16. Bosnjak B, et al. 2021. Front Immunol. 12:772240. PubMed
  17. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  18. Souza SP, et al. 2021. PLoS Pathog. 17:e1010081. PubMed
  19. Pandit M, et al. 2021. Eur J Immunol. 51:1461. PubMed
  20. Wang Y, et al. 2020. Virol Sin. 36:122. PubMed
  21. Waight JD, et al. 2018. Cancer Cell. 33:1033. PubMed
  22. Truckenbrod EN, et al. 2021. Elife. 10:. PubMed
  23. Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed
  24. Jackson C, et al. 2016. Clin Cancer Res. 22: 1161 - 1172. PubMed
  25. Lei F, et al. 2012. J Vis Exp. 63: 3986. PubMed
  26. Olguín J, et al. 2015. Microbes Infect. 17: 586-595. PubMed
RRID
AB_315303 (BioLegend Cat. No. 503809)
AB_315304 (BioLegend Cat. No. 503810)

Antigen Details

Structure
Cytokine; 15-30 kD (Mammalian)
Bioactivity
Proliferation of T lymphocytes, B cells, anti-inflammatory, hematopoiesis, tumor surveillance
Cell Sources
T cells
Cell Targets
T cells, B cells, NK cells, LAK cells, monocytes, macrophages, oligodendrocytes
Receptors
High affinity heterotrimer of IL-2Rα/β/γ, intermediate affinity homodimer IL-2Rα (CD25; p55; Tac) and heterodimer IL-2Rβ (CD122)/γ; γ-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R
Cell Type
Tregs
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Taniguchi T, et al. 1993. Cell 73:5.
3. Nistico G. 1993. Prog. Neurobiol. 40:463.
4. Waldmann T, et al. 1993. Ann. NY Acad. Sci. 685:603.

Regulation
Upregulated by NFAT; downregulated by TCF-8, CIF (colostrum inhibitory factor)
Gene ID
16183 View all products for this Gene ID
UniProt
View information about IL-2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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