APC anti-mouse IFN-γ R β chain Antibody

Pricing & Availability
Clone
MOB-47 (See other available formats)
Other Names
IFN-γ Receptor β chain, IFN-γ AF-1
Isotype
Armenian Hamster IgG
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Product Citations
publications
MOB-47_APC_IFN-yRB-Antibody_061918
Murine lymphoma cell line A20 was stained with IFN-γR β chain (clone MOB-47) APC (filled histogram) or Armenian Hamster IgG APC isotype control (open histogram).
  • MOB-47_APC_IFN-yRB-Antibody_061918
    Murine lymphoma cell line A20 was stained with IFN-γR β chain (clone MOB-47) APC (filled histogram) or Armenian Hamster IgG APC isotype control (open histogram).
See APC spectral data
Cat # Size Price Quantity Avail. Save
113605 25 µg £74
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113606 100 µg £161
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Description

IFN-γ receptor β chain is also known as IFN-γ AF-1. This 60-65 kD class II cytokine receptor pairs with CDw119 to form the IFN-γ receptor. CDw119 serves as the IFN-γ-binding chain and associates with the IFN-γ β chain which is required for receptor signaling. The extracellular portion of both the IFN-γ receptor α and β chains must be species matched. The IFN-γ receptor β chain is expressed at low levels on T and B cells, NK cells, monocytes/ macrophages, and fibroblasts. Binding of IFN-γ induces receptor dimerization, internalization, Jak1 and Jak2 protein kinase activation and, ultimately, STAT1 activation. IFN-γ initiates and regulates a variety of immune responses.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse IFN-γR β chain protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1 and immunoprecipitation1. For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 113604) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)).

Application References

(PubMed link indicates BioLegend citation)
  1. Bach EA, et al. 1995. Science 270:1215.
RRID
AB_2734170 (BioLegend Cat. No. 113605)
AB_2783052 (BioLegend Cat. No. 113606)

Antigen Details

Structure
Class II cytokine receptor family, associates with CDw119, 60-65 kD, AF-1
Distribution

T and B cells, NK cells, monocytes, macrophages, granulocytes, fibroblasts

Function
Regulates immune response, upregulates MHC class I expression
Ligand Receptor
IFN-γ
Cell Type
T cells, B cells, NK cells, Monocytes, Macrophages
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Bach EA, et al. 1995. Science 270:1215. 
2. Ansel KM, et al. 2003. Nat. Immunol. 4:616.

Gene ID
NA
UniProt
View information about IFN-g R b chain on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/19/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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