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APC anti-mouse CD8b Antibody

Pricing & Availability
Clone
YTS156.7.7 (See other available formats)
Regulatory Status
RUO
Other Names
Lyt-3, Ly-3
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
YTS156.7.7_APC_CD8b_Antibody_FC_111313.jpg
C57BL/6 mouse splenocytes were stained with CD8b (clone YTS156.7.7) APC (filled histogram) or rat IgG2b, κ APC isotype control (open histogram).
  • YTS156.7.7_APC_CD8b_Antibody_FC_111313.jpg
    C57BL/6 mouse splenocytes were stained with CD8b (clone YTS156.7.7) APC (filled histogram) or rat IgG2b, κ APC isotype control (open histogram).
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
126613 25 µg £70
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126614 100 µg £183
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Description

CD8b is the 32 kD β chain of CD8, also known as Lyt-3 or Ly-3. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. McNical AM, et al. 2007. Eur. J. Immunol. 37:1634.
Product Citations
  1. Weigelin B, et al. 2015. Proc Natl Acad Sci U S A. 112:7551. PubMed
  2. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  3. Goswami M, et al. 2019. Biomed Opt Express. 10:151. PubMed
  4. Overacre-Delgoffe AE, et al. 2017. Cell. 169:1130. PubMed
  5. Aulova KS, et al. 2022. Molecules. 27:. PubMed
  6. Reed-Geaghan EG, et al. 2020. J Exp Med. 217:00:00. PubMed
  7. Wang Y, et al. 2022. Nat Cell Biol. 24:230. PubMed
RRID
AB_2562774 (BioLegend Cat. No. 126613)
AB_2562775 (BioLegend Cat. No. 126614)

Antigen Details

Structure
Ig superfamily, CD8β chain, 34 kD
Distribution

Majority of thymocytes, cytotoxic T cell subsets

Function
Co-receptor of TCR
Ligand/Receptor
MHC class I
Cell Type
T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1.Barclay A, et al. 1997. The Leukocyte antigen Facts Book Academic Press.
2. Zamoyska R. 1994. Immunity 1:243-246.
3. Ellmeier W, et al. 1999. Annu. Rev Immunol 17:523.
4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503.

Gene ID
12526 View all products for this Gene ID
UniProt
View information about CD8b on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/15/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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