APC anti-mouse CD70 Antibody

Pricing & Availability
FR70 (See other available formats)
Regulatory Status
Other Names
Ki-24, CD27-ligand, TNFSF7
Rat IgG2b, κ
Ave. Rating
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Product Citations
B cell lymphoma cell line, A20, was stained with CD70 (clone FR70) APC (filled histogram) or rat IgG2b, κ isotype APC control (open histogram).
  • FR70_APC_CD70_Antibody_091018
    B cell lymphoma cell line, A20, was stained with CD70 (clone FR70) APC (filled histogram) or rat IgG2b, κ isotype APC control (open histogram).
Compare all formats See APC spectral data
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104609 25 µg £72
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104610 100 µg £140
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CD70 is a type II transmembrane protein. It is a member of the TNF superfamily, expressed on activated B cells, activated dendritic cells, and some activated T cells (low levels). CD70 interacts with CD27 to promote T-B cell cross-stimulation and co-stimulate B cell proliferation and immunoglobulin production. Cells expressing CD70 can co-stimulate T cell proliferation and enhance the production of cytokines. The FR70 antibody blocks CD70 binding to CD27.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Host Species
BALB/c mouse B lymphoma A20.2J
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro blocking of CD70-CD27 binding and inhibition of T and NK cell responses1-3 , and immunohistochemistry4 of aceton-fixed frozen sections. For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 104605/104606) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 104604) or biotinylated anti-rat IgG (Cat. No. 405402) second step, followed by SAv-PE (Cat. No. 405204)). To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 104608).

Application References

(PubMed link indicates BioLegend citation)
  1. Oshima H, et al. 1998. Int. Immunol. 10:517. (Block IP)
  2. Akiba HH, et al. 1999. J. Immunol. 162:7058. (Block)
  3. Takeda K, et al. 2000. J. Immunol. 164:1741. (Block)
  4. Henke A, et al. 2000. J. Virol. 74:4284. (IHC)
  5. Brown SL, et al. 2007. J. Clin. Invest. 117:258.
AB_2750464 (BioLegend Cat. No. 104609)
AB_2750465 (BioLegend Cat. No. 104610)

Antigen Details

TNF/NGF superfamily, 30-33 kD

Activated B cells and some activated T cells

T-B cell cross-stimulation
Cell Type
B cells, T cells
Biology Area
Immunology, Costimulatory Molecules
Molecular Family
CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Tesselaar K, et al. 1997. J. Immunol. 159:4959.
3. Akiba H, et al. 1999. J. Immunol. 162:7058.

Gene ID
21948 View all products for this Gene ID
View information about CD70 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/10/2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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