APC anti-mouse CD49e Antibody

Pricing & Availability
5H10-27(MFR5) (See other available formats)
Other Names
α5 integrin, VLA-5 α chain, Integrin α5, Fnra, Itga5
Rat IgG2a, κ
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Product Citations
C57BL/6 mouse bone marrow cells were stained with CD49e (clone 5H10-27) APC (filled histogram) or Rat IgG2a, κ APC isotype control (open histogram).
  • 5H10-27_APC_CD49e_Antibody_07122018
    C57BL/6 mouse bone marrow cells were stained with CD49e (clone 5H10-27) APC (filled histogram) or Rat IgG2a, κ APC isotype control (open histogram).
See APC spectral data
Cat # Size Price Quantity Avail. Save
103813 25 µg £55
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103814 100 µg £130
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CD49e is a 135 kD protein, also known as α5 integrin or VLA-5 α chain. It is a member of the integrin family, expressed on thymocytes, splenic B cells, activated T cells, and mast cells. CD49e associates with CD29 (integrin β1 chain) to form the fibronectin receptor (VLA-5). CD49e plays a critical role in both adhesion and T cell costimulation. The primary ligand for CD49e/CD29 (VLA-5) is fibronectin. The 5H10-27(MFR5) antibody has been shown to block CD49e mediated interactions and promote the in vitro stimulation of CD8+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Host Species
(C57BL/6 x A/J)F1 mouse mast cell line
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining6,7 of acetone-fixed frozen sections, blocking of cell-cell adhesion1-5, inhibition of TNF-ß1 costimulated T cell proliferation3, and costimulation of T cell proliferation by cross-linked 5H10-27 antibody3. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103817 and 103818).

Application References

(PubMed link indicates BioLegend citation)
  1. Kinashi T, et al. 1994. Blood Cells 20:25. (Block)
  2. Halvorson MJ, et al. 1995. J. Immunol. 155:4567. (Block)
  3. Rich S, et al. 1996. J. Immunol. 157:2916. (Block Costim))
  4. Uhlenkott CE, et al. 1996. Clin. Exp. Metastasis 14:125. (Block)
  5. Schultz JF, et al. 1995. J. Biol. Chem. 270:11522. (Block)
  6. Yang JT, et al. 1996. Mol. Biol. Cell. 7:1737. (IHC)
  7. Cuklerman E, et al. 2001. Science 294:1708. (IHC)
  8. Jia W, et al. 2005. Blood 106:3854. (FC)
AB_2750076 (BioLegend Cat. No. 103813)
AB_2750077 (BioLegend Cat. No. 103814)

Antigen Details

Integrin family, associates with integrin β1, 135 kD

Thymocytes, activated T cells, mast cells

Adhesion, costimulation
Cell Type
Mast cells, Mesenchymal Stem Cells, T cells, Thymocytes, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kinashi T, et al. 1994 Blood Cells 20:25.
3. Hemler ME. 1990. Annu. Rev. Immunol. 8:365.

Gene ID
16402 View all products for this Gene ID
View information about CD49e on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/16/2018

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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