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APC anti-mouse CD105 Antibody

Pricing & Availability
Clone
MJ7/18 (See other available formats)
Regulatory Status
RUO
Other Names
Endoglin
Isotype
Rat IgG2a, κ
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Product Citations
publications
MJ7slash18_APC_040810
Mouse endothelial cell line bEnd.3 stained with MJ7/18 APC
  • MJ7slash18_APC_040810
    Mouse endothelial cell line bEnd.3 stained with MJ7/18 APC
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120413 25 µg £62
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120414 100 µg £146
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Description

CD105 is a 90 kD homodimeric type I integral membrane glycoprotein, also known as endoglin. It is expressed on endothelial cells (especially on angiogenic endothelial cells) and upregulated by hypoxia, activated monocytes, macrophages, bone marrow stromal cells, and some cytotrophoblasts. CD105 is a receptor for TGF-β1, TGF-β3 and modulates TGF-β signaling by interacting with TGF-β receptors I and/or II. CD105 also binds other growth factors such as actvin A, BMP-2, and BMP-7. CD105 has been show to be a useful marker for identifying proliferating endothelium involved in tumor angiogenesis and can be used for tumor imaging and prognosis, and has therapeutic potential for some solid tumors and other angiogenic diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Inflamed mouse skin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications include: immunoprecipitation, Western blotting, and immunofluorescence histochemistry or immunohistochemistry of acetone-fixed frozen sections2-4.

Application References

(PubMed link indicates BioLegend citation)
  1. Ge AZ and Butcher EC. 1994. Gene 138:201.
  2. Baluk P, et al. 2003. Am. J. Pathol. 163:1801. (IHC)
  3. Takahashi T, et al. 2003. Mol. Cell Biol. 23:1817. (IHC)
  4. Savinov AY, et al. 2003. J. Exp. Med. 197:643. (IHC)
Product Citations
  1. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  2. Huang X, et al. 2021. Stem Cells Int. 2021:8138374. PubMed
  3. Lai J, et al. 2021. Stem Cell Res. 55:102477. PubMed
  4. Li Z, et al. 2022. Elife. 11:. PubMed
  5. Hughes RM, et al. 2020. Cancer Research. 79(14):3636-3650.. PubMed
  6. Liu H, et al. 2022. Nat Commun. 13:3684. PubMed
  7. Galindo C, et al. 2014. J Am Heart Assoc. 3:773. PubMed
  8. Zhu L, et al. 2021. Stem Cell Res Ther. 12:408. PubMed
  9. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  10. Formes H, et al. 2021. iScience. 24:103092. PubMed
  11. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  12. Nanou A, et al. 2021. Cell Reports. 35(8):109168. PubMed
  13. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  14. Yang P et al. 2019. Cell Syst. 8(5):427-445 . PubMed
  15. Nenasheva T, et al. 2017. PLoS One. 12(6):e0178983. PubMed
  16. Yamazaki M, et al. 2021. Biomedicines. 9:. PubMed
  17. Jin Y, et al. 2020. PeerJ. 8:e10374. PubMed
  18. Wang X, et al. 2018. Nat Commun. 9:551. PubMed
  19. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  20. Yang X, et al. 2021. Bioact Mater. 3150:6. PubMed
  21. Fan X, et al. 2020. Acta Physiol (Oxf). 230:e13475. PubMed
  22. Sanz-Ros J, et al. 2022. Sci Adv. 8:eabq2226. PubMed
  23. Woods B, et al. 2019. Clin Cancer Res. 25:5901. PubMed
  24. Shao Y, et al. 2017. Endocrinology. 158:2722. PubMed
  25. Tu Z, et al. 2022. Cell Rep. 40:111072. PubMed
  26. Lucotti S, et al. 2019. J Clin Invest. 130:1845. PubMed
  27. Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
  28. Zhu Q, et al. 2021. J Am Heart Assoc. 10:e023491. PubMed
RRID
AB_2277915 (BioLegend Cat. No. 120413)
AB_2277914 (BioLegend Cat. No. 120414)

Antigen Details

Structure
Type I integral membrane protein, homodimer, TGF-β type III receptor family member
Distribution

Endothelial cells, activated monocytes, macrophages, stromal cells, some cytotrophoblast

Function
Suppresses TGF-β signaling, angiogenesis
Ligand/Receptor
TGF-β1, TGF-β3
Cell Type
Endothelial cells, Macrophages, Mesenchymal Stem Cells, Monocytes
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Gougos A and M. Letarte 1988. J. Immunol. 141:1925.
2. Cheifetz S, et al. 1992. J. Bio. Chem. 267:19027.
3. Barbara NP, et al. 1999. J. Bio. Chem. 274:584.
4. Lastres P, et al. 1992. Eur. J. Immunol. 22:393.
5. Duff S, et al. 2003. FASEB J. 17:984.
6. Warrington K, et al. 2005. Anticancer Res. 25:185.

Gene ID
13805 View all products for this Gene ID
UniProt
View information about CD105 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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