APC anti-human HLA-F Antibody

Pricing & Availability
Clone
3D11/HLA-F (See other available formats)
Other Names
HLAF, CDA12, HLA-5.4, HLA-CDA12
Isotype
Mouse IgG1, κ
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Product Citations
publications
3D11-HLA-F_APC_HLA-F_Antibody_101518
PMA+Ionomycin-stimulated (6 hours) human peripheral blood lymphocytes was stained with CD20 FITC and HLA-F (clone 3D11/HLA-F, left) APC or mouse IgG1, κ isotype control (right) APC.
  • 3D11-HLA-F_APC_HLA-F_Antibody_101518
    PMA+Ionomycin-stimulated (6 hours) human peripheral blood lymphocytes was stained with CD20 FITC and HLA-F (clone 3D11/HLA-F, left) APC or mouse IgG1, κ isotype control (right) APC.
See APC spectral data
Cat # Size Price Quantity Avail. Save
373207 25 tests £81
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373208 100 tests £188
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Description

A 42kD member of non-classical or class Ib human MHC family. It exhibits low allelic polymorphism and shorter cytoplasmic tail due to absence of exon seven. It is expressed irrespective of bound peptide. It is primarily found intracellularly on Endoplasmic Reticulum and Golgi membrane and its surface expression increases in correlation with classical MHC-I up regulation in activated monocytes, B, T, and NK cells. It is involved in inflammatory responses. It stabilizes the unstable MHC-I Open Conformers (OC) and facilitates uptake of extracellular antigen for cross presentation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Complex mixed with IFA, complex without adjuvant.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Ishitani A, et al. 2003. J. Immunol. 171:1376.
RRID
AB_2783287 (BioLegend Cat. No. 373207)
AB_2783288 (BioLegend Cat. No. 373208)

Antigen Details

Structure
42 kD Single pass type I membrane protein, heterodimer of an alpha and a Beta chain.
Distribution

B, NK, monocyte and T cells.

Function
MHC-I OC stabilization, antigen cross presentation.
Ligand Receptor
KIR3DS1, KIR3DS4, KIR3DL2, ILT2, ILT4, TAP.
Cell Type
B cells, NK cells, T cells
Biology Area
Immunology
Molecular Family
MHC Antigens
Antigen References
  1. Goodridge JP, et al. 2013. J. Immunol. 191:1567.
  2. Lepin EJ, et al. 2000. Eur. J. Immunol. 30:3552.
  3. Burian A,et al. 2016. PLoS One 11:e0163297.
  4. Lee N, et al. 2010. Eur. J. Immunol. 40:2308.
Gene ID
3134 View all products for this Gene ID
UniProt
View information about HLA-F on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/15/2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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