- LN2 (See other available formats)
- Regulatory Status
- HCDM listed
- Other Names
- MHC Class II associated invariant chain, invariant chain, Ii, MHC class II chaperone, MIF receptor
- Mouse IgG1, κ
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- Product Citations
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CD74 is a type II transmembrane glycoprotein also known as MHC class II associated invariant chain, invariant chain, Ii, MHC class II chaperone, and MIF receptor. CD74 exists in four isoforms with molecular masses of 33, 35, 41, and 43 kD, depending on genetic splicing. CD74 is primarily expressed on antigen presenting cells, including B cells, monocytes/macrophages, dendritic cells, and Langerhans cells. It is also expressed by activated T cells and activated endothelial and epithelial cells as well as carcinomas of lung, renal, gastric and thymic origin. The primary function of CD74 is intracellular sorting of MHC class II molecules and regulation of exogenous peptide loading onto MHC class II. It is also involved in the modulation of B cell differentiation and positive selection of CD4+ T cells. It has been reported that CD74 binds MIF (macrophage migration inhibitory factor) and signals through CD44 to regulate innate and adaptive immunity. It is also reported that H. pylori infection occurs through urease B binding of CD74 on gastric epithelial cells, inducing gastric epithelial cell apoptosis, NF-κB activation, and IL-8 production.Product Details
- Human, African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Host Species
- SU-DHL-4 cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
Red Laser (633 nm)
- Application Notes
Clone LN2 is reactive with an epitope residing within 60 amino acids of the extracytoplasmic, COOH terminus of the protein.3
Additional reported applications (for the relevant formats) include: immunohistochemical staining1,2 of frozen sections and formalin-fixed paraffin-embedded sections1,2, immunoprecipitation1, and immunofluorescence4.
(PubMed link indicates BioLegend citation)
- Epstein AL, et al.1984. J. Immunol. 133:1028. (IHC, IP)
- Marder RJ, et al. 1985. Lab. Invest. 52:497. (IHC)
- Wraight CJ, et al. 1990. J. Biol. Chem. 265:5787.
- Leng L, et al. 2003. J. Exp. Med. 197:1467. (IF)
- Product Citations
AB_2564388 (BioLegend Cat. No. 326811)
AB_2564389 (BioLegend Cat. No. 326812)
- Type II transmembrane glycoprotein, 33, 35, 41, 43 kD
B cells and other antigen presenting cells, activated T cells, activated endothelial and epithelial cells, and certain carcinomas
- Regulates loading of exogenous peptides onto MHC class II and B cell differentiation, involved in positive selection of CD4+ T cells.
- HLA-DR, MIF, H. pylori urease B, associates with CD44
- Cell Type
- Antigen-presenting cells, B cells, Endothelial cells, Epithelial cells, T cells, Tregs
- Biology Area
- Molecular Family
- CD Molecules, MHC Antigens
- Antigen References
1. Moldenhauer G, et al. 1999. Immunology 96:473.
2. Shi X, et al. 2006. Immunity 25:595.
3. Beswick EJ, et al. 2006. Infect. Immun. 74:1148.
4. Zola H,et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication
- Gene ID
- 972 View all products for this Gene ID
- View information about CD74 on UniProt.org
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